The EMPIAR-PDBj team at Osaka University assists Asian EM researchers with the transfer of big EM image data to EMPIAR. Instead of sending the data directly to the EBI (UK) via the internet, hard drives can also be sent to Osaka University by postal mail or via a courier service. As an alternative, internet transfer to our server in Osaka is also available. If you would like to take advantage of our submission services, please contact us first by e-mail before sending the data to us.
We have seen a large number of SARS-CoV-2 structures being determined rapidly and deposited into the PDB and EMDB, which provides a starting point for structure-guided drug discovery. In order to validate and reanalyze the data by the cryo-EM community, we would like to make the following urgent appeal. a) If you have deposited any cryo-EM structures related to SARS-CoV-2 to the EMDB (and possibly the PDB), please deposit the raw data to EMPIAR. b) If you have collected cryo-EM data related to SARS-CoV-2 but have not yet deposited a structure, please consider depositing the raw data to EMPIAR, so that the cryo-EM community could contribute to processing and model-building.
Release date | Imageset | Title | Authors and references | Size | Resolution |
---|---|---|---|---|---|
2021-10-18 | Cryo electron tomography of FIB-milled lamella of human DLD-1 cells [3 tilt series in MRC format] | Schuller AP, Wojtynek M, Mankus D, Tatli M, Kronenberg-Tenga R, Regmi SG, Dip PV, Lytton-Jean AKR, Brignole EJ, Dasso M, Weis K, Medalia O, Schwartz TU [Pubmed: 34646014] [DOI: 10.1038/s41586-021-03985-3] |
8.0 GB | 33.0 - 39.0 Å | |
2021-10-18 | Cryo electron tomography of FIB-milled lamella of human DLD-1 cells [3 tilt series in MRC format] | Schuller AP, Wojtynek M, Mankus D, Tatli M, Kronenberg-Tenga R, Regmi SG, Dip PV, Lytton-Jean AKR, Brignole EJ, Dasso M, Weis K, Medalia O, Schwartz TU [Pubmed: 34646014] [DOI: 10.1038/s41586-021-03985-3] |
8.0 GB | 33.0 - 39.0 Å | |
2015-11-04 | In vitro assembled bacteriophage phi6 polymerase complex [stack of 798 particles in MRC format] | Ilca SL, Kotecha A, Sun X, Poranen MP, Stuart DI, Huiskonen JT [Pubmed: 26534841] [DOI: 10.1038/ncomms9843] |
8.1 GB | 7.9 Å | |
2023-05-12 | Quantification of gallium cryo-FIB milling damage in biological lamella [multiple data sets in MRC format] | Lucas BA, Grigorieff N [Pubmed: 37216561] [DOI: 10.1073/pnas.2301852120] |
8.1 GB | — | |
2022-10-17 | Cryo-electron tomography of microtubules assembled from purified porcine brain tubulin in the presence of GTP [4 reconstructed volumes in MRC format] | Guyomar C, Bousquet C, Ku S, Heumann J, Guilloux G, Gaillard N, Heichette C, Duchesne L, Steinmetz MO, Gibeaux R, Chrétien D [Pubmed: 36503602] [DOI: 10.7554/eLife.83021] |
8.4 GB | 25.0 Å | |
2024-01-16 | REEP3 and REEP4 determine the tubular morphology of the endoplasmic reticulum during mitosis [multiple data sets in DM4 and TIFF formats] | Belevich I, Jokitalo E [Pubmed: 30995177] [DOI: 10.1091/mbc.e18-11-0698] |
8.7 GB | — | |
2021-05-21 | Tilt series of dividing vegetative and sporulating cells of Bacillus subtilis from the manuscript - Khanna et al., 2021 [multiple data sets in MRC format] | Khanna K, Lopez-Garrido J, Sugie J, Pogliano K, Villa E [Pubmed: 34018921] [DOI: 10.7554/eLife.62204] |
8.7 GB | — | |
2015-04-24 | Sub-tomogram average of a mammalian F-type ATP synthase monomer [multiple data sets in DM4 format] | Jiko C, Davies KM, Shinzawa-Itoh K, Tani K, Maeda S, Mills DJ, Tsukihara T, Fujiyoshi Y, Kuehlbrandt W, Gerle C [Pubmed: 25815585] [DOI: 10.7554/eLife.06119] |
8.9 GB | 24.0 Å | |
2017-12-18 | CryoET of Mtb 20S proteasome single particle [multiple data sets in MRC format] | Noble AJ, Dandey VP, Wei H, Brasch J, Chase J, Acharya P, Tan YZ, Zhang Z, Kim LY, Scapin G, Rapp M, Eng ET, Rice MJ, Cheng A, Negro CJ, Shapiro L, Kwong PD, Jeruzalmi D, des Georges A, Potter CS, Carragher B [Pubmed: 29809143] [DOI: 10.7554/eLife.34257] |
9.1 GB | — | |
2021-06-09 | 120kV MicroED structure of FUS (37-42) SYSGYS solved from merged datasets at 0.60 A [8 diffraction images in SMV format] | Zhou H, Luo F, Luo Z, Li D, Liu C, Li X [Pubmed: 31334636] [DOI: 10.1021/acs.analchem.9b01162] |
9.2 GB | 0.6 Å | |
2021-06-09 | 200kV MicroED structure of FUS (37-42) SYSGYS solved from merged datasets at 0.65 A [8 diffraction images in SMV format] | Zhou H, Luo F, Luo Z, Li D, Liu C, Li X [Pubmed: 31334636] [DOI: 10.1021/acs.analchem.9b01162] |
9.5 GB | 0.65 Å | |
2022-10-07 | Tilt series of SARS-CoV-2 spike-bearing virus-like particles (VLPs) interacting with hACE2-bearing extracellular vesicles (tEVs), showing various intermediate states of the SARS-CoV-2 spike protein [6 tilt series in MRC format] | Marcink TC, Porotto M, des Georges A, Moscona A [Pubmed: 35984891] [DOI: 10.1126/sciadv.abo3153] |
9.6 GB | — | |
2021-06-18 | cryo-FIB and cryo-ET study of ribosome and polysome structures in E. coli [3 tilt series in MRC format] | Xiang YJ, Chang YJ, Liu J, Jacobs-Wagner C [Pubmed: 34186018] [DOI: 10.1016/j.cell.2021.05.037] |
9.7 GB | 20.0 - 27.0 Å | |
2022-03-28 | Representative data from Near-native state imaging by cryo-soft-X-ray tomography reveals remodelling of cytoplasmic vesicles and mitochondria during HSV-1 infection [14 reconstructed volumes in MRC format] | Nahas KLN, Connor VC, Scherer KM, Kaminski CF, Harkiolaki M, Crump CM, Graham SC [DOI: 10.1101/2021.10.11.463900] |
9.9 GB | — | |
2021-11-26 | Reconstructed cryo soft X-ray tomography dataset of a treated NIH-3T3 cell with corresponding correlated cryo-3D-SIM channels [300 reconstructed volumes in TIFF format] | Groen J, Pereiro E [Pubmed: 34909150] [DOI: 10.1039/d1sc04183e] |
10.1 GB | — | |
2018-07-27 | Single particle cryo-EM dataset of the flexible and variable oligomeric state complex AP-1:Arf1:tetherin-HIV-Nef [stack of 53841 particles in MRCS format] | Morris KL, Buffalo CZ, Hurley JH [Pubmed: 30053425] [DOI: 10.1016/j.cell.2018.07.004] |
10.1 GB | 3.73 - 4.27 Å | |
2021-11-16 | Subcellular architecture collodaria photosymbiosis [7 multi-frame micrographs composed of 1000 frames each in TIFF format] | Decelle JD [Pubmed: 34499794] [DOI: 10.1111/1462-2920.15766] |
10.3 GB | — | |
2022-05-03 | In situ single particle classification reveals distinct 60S maturation intermediates in cells [multiple data sets in MRC format] | Lucas BA, Zhang K, Loerch S, Grigorieff N [DOI: 10.1101/2022.04.10.487797] |
10.5 GB | — | |
2014-06-19 | Cryo-electron tomography average of an C1-IgG complex [27 class averages in MRC format] | Diebolder CA, Beurskens FJ, de Jong RN, Koning RI, Strumane K, Lindorfer MA, Voorhorst M, Ugurlar D, Rosati S, Heck AJR, van de Winkel JGJ, Wilson IA, Koster AJ, Taylor RP, Ollmann-Saphire E, Burton DR, Schuurman J, Gros P, Parren PWHI [Pubmed: 24626930] [DOI: 10.1126/science.1248943] |
10.8 GB | 66.0 Å | |
2023-03-17 | Serial section electron tomography of a Leishmania haptomonad-like cell attached to plastic [718 multi-frame micrographs composed of 1 frames each in MRC format] | Yanase R, Sunter JD [DOI: 10.1101/2022.10.28.514187] |
10.8 GB | — | |
2017-11-30 | FIB-SEM of a dividing cell at 11.2 min after anaphase onset [777 multi-frame micrographs composed of 1 frames each in TIFF format] | Otsuka S, Steyer AM, Schorb M, Heriche JK, Hossain MJ, Sethi S, Kueblbeck M, Schwab Y, Beck M, Ellenberg J [DOI: 10.1038/s41594-017-0001-9] |
11.0 GB | — | |
2021-07-07 | Locating Macromolecular Assemblies in Cells by 2D Template Matching with cisTEM [multiple data sets in MRC format] | Lucas BA, Himes BA, Xue L, Grant T, Mahamid J, Grigorieff N [Pubmed: 34114559] [DOI: 10.7554/eLife.68946] |
11.0 GB | 20.0 Å | |
2016-01-27 | Cryo-electron tomogram of host-free Chlamydia trachomatis with type III secretion system [1 class averages in MRC format] | Nans A, Kudryashev M, Saibil HR, Hayward RD [Pubmed: 26656452] [DOI: 10.1038/ncomms10114] |
11.6 GB | 33.0 Å | |
2020-09-11 | Human Parainfluenza Virus Fusion Complex Glycoproteins Imaged In Action On Authentic Viral Surfaces [2 tilt series in MRC format] | Marcink TC, Wang T, des Georges A, Porotto M, Moscona A [Pubmed: 32956394] [DOI: 10.1371/journal.ppat.1008883] |
11.6 GB | 17.18 Å | |
2023-01-03 | Cryo electron tomography of Ca. L. ossiferum [multiple data sets in MRC format] | Wollweber F, Xu J [Pubmed: 36544020] [DOI: 10.1038/s41586-022-05550-y] |
11.6 GB | 11.7 - 24.5 Å |