EMPIAR-10044 - In vitro assembled bacteriophage phi6 polymerase complex [stack of 798 particles in MRC format]

EMPIAR-10044

In vitro assembled bacteriophage phi6 polymerase complex [stack of 798 particles in MRC format]

Publication:

Localized reconstruction of subunits from electron cryomicroscopy images of macromolecular complexes

Ilca SL, Kotecha A, Sun X, Poranen MP, Stuart DI, Huiskonen JT

Nat Commun 6 (2015) 8843

PMID: 26534841

DOI: doi:10.1038/ncomms9843

Related EMDB entry:

EMD-3187

Deposited:

2015-10-30

Released:

2015-11-04

Last modified:

2015-11-04

Imageset size:

8.05 GB

Imageset DOI:

10.6019/EMPIAR-10044

Experimental metadata:

Download xml json

Contains:

picked particles - multiframe - processed

Image Sets

1. Extracted particle stacks of bacteriophage phi6 polymerase complex

Category:

picked particles - multiframe - processed

Image format:

MRC

No. of images or tilt series:

798

Image size:

(480, 480)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.35, 1.35)

Details:

Particle extracted and normalized in Relion. Contrast has been inverted. Each of 798 MRCS files is a stack of individual particle images (not multiple frames of the same particle image).

Files:

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Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

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Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)

The 2023 Nucleic Acids Research Database Issue and the online molecular biology database collection.

Rigden DJ, Fernández XM. (2023)

EMPIAR: the Electron Microscopy Public Image Archive.

Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)

CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM.

Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)

Structure of a RecT/Redβ family recombinase in complex with a duplex intermediate of DNA annealing.

Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)

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