EMPIAR-10589
cryo-FIB and cryo-ET study of ribosome and polysome structures in E. coli [3 tilt series in MRC format]
Publication:

Solvent quality and chromosome folding in Escherichia coli

Xiang YJ, Chang YJ, Liu J, Jacobs-Wagner C

(---)

Related EMDB entry:
Deposited:
2020-10-17
Released:
2021-06-18
Last modified:
2021-06-18
Imageset size:
9.65 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • tilt series
1. Unaligned tilt series of cryo-FIB milling E. coli cells
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
3
Image size:
(3838, 3710)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(5.457, 5.457)
Details:
1. Imaging parameters: Titan Krios, K2, ~100 total electron dose per angstrom square, bi-directional from ~0 degree.
2. Ecoli_001.mrc: collect positive angle images first, then negative angle images.
3. Ecoli_002.mrc and Ecoli_003.mrc: collect negative angle images first, then positive angle images.
4. The corresponding .tlt files are the tilt angles after compensate the tilt between the lamella and the grid.
Files:
Loading...
Files:
Loading...

Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)
Rigden DJ, Fernández XM. (2023)
Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)
Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)
Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)