EMPIAR-10589
cryo-FIB and cryo-ET study of ribosome and polysome structures in E. coli [3 tilt series in MRC format]
Publication:

Solvent quality and chromosome folding in Escherichia coli

Xiang YJ, Chang YJ, Liu J, Jacobs-Wagner C

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Related EMDB entry:
Deposited:
2020-10-17
Released:
2021-06-18
Last modified:
2021-06-18
Imageset size:
9.65 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • tilt series
1. Unaligned tilt series of cryo-FIB milling E. coli cells
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
3
Image size:
(3838, 3710)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(5.457, 5.457)
Details:
1. Imaging parameters: Titan Krios, K2, ~100 total electron dose per angstrom square, bi-directional from ~0 degree.
2. Ecoli_001.mrc: collect positive angle images first, then negative angle images.
3. Ecoli_002.mrc and Ecoli_003.mrc: collect negative angle images first, then positive angle images.
4. The corresponding .tlt files are the tilt angles after compensate the tilt between the lamella and the grid.
Files:
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