EMPIAR-13504
The cryo-EM structure of porcine serum MGAM [multiple data sets in MRCS and TIFF formats]
Publication:

Porcine serum maltase-glucoamylase: structure, kinetics, and inhibition

Watanabe K, Tagami T, Biwa C, Kawasaki M, Adachi N, Moriya T, Senda T, Okuyama M

Journal of enzyme inhibition and medicinal chemistry 41 (2026)

PMID: 41534875

Related EMDB entry:
Deposited:
2026-03-04
Released:
2026-06-17
Last modified:
2026-06-17
Imageset size:
2.67 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. [EMD-62652] The cryo-EM structure of porcine serum MGAM
Category:
micrographs - multiframe
Image format:
MRCS
No. of images or tilt series:
919
Image size:
(4096, 4096)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.88, 0.88)
Details:
- Acceleration voltage (kV): 200
- Pixel size (A/pix): 0.88
- Spherical aberration (Cs): 2.7
- Average electron dose per image (e-/A2): 50
- Microscope: Talos Arctica G2
- Detector: Falcon3EC (4k × 4k)
- Nominal Defocus (nm): -1000 to -2500
- Number of frames per image: 50
Files:
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2. [EMD-62653] The cryo-EM structure of porcine serum MGAM bound with Acarviosyl-maltotriose
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
6018
Image size:
(4092, 5760)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.83, 0.83)
Details:
- Acceleration voltage (kV): 300
- The pixel size in the image header (1 Å/px) is wrong. 0.83 Å/px is the calibrated value.
- Spherical aberration (Cs): 2.7
- Average electron dose per image (e-/A2): 49
- Microscope: Krios G3i
- Detector: Gatan K3 (6k × 4k)
- Nominal Defocus (nm): -1000 to -1800
- Number of frames per image: 49
- The first frames in all movies should be excluded from processing.
Files:
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