- Acceleration voltage (kV): 300 - The pixel size in the image header (1 Å/px) is wrong. 0.83 Å/px is the calibrated value. - Spherical aberration (Cs): 2.7 - Average electron dose per image (e-/A2): 49 - Microscope: Krios G3i - Detector: Gatan K3 (6k × 4k) - Nominal Defocus (nm): -1000 to -1800 - Number of frames per image: 49 - The first frames in all movies should be excluded from processing.