EMPIAR-12914
CryoEM dataset of M. mazei topoisomerase VI(A:E342Q)-minicircle DNA complex [26728 multi-frame micrographs composed of 1233 frames each in EER format]
Publication:

Supercoiled DNA recognition and cleavage control in topoisomerase VI

Richman DE, Wendorff TJ, Rashid F, Beck C, Yan Q, Johnson HR, Eckerty RA, Fogg JM, Baker ML, Zechiedrich L, Berger JM

Nature communications 17 (2026)

PMID: 41698922

Related EMDB entry:
Deposited:
2025-07-30
Released:
2026-03-04
Last modified:
2026-04-07
Imageset size:
14.07 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. Unaligned eer micrographs of M mazei Top6(A:E342Q)-306 bp minicircle DNA-ADPNP complex
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
26728
Image size:
(4096, 4096)
Pixel type:
BIT
Pixel spacing:
(0.93, 0.93)
Details:
This dataset was collected with no stage tilt. Data were collected at the Beckman Center for CryoEM at Johns Hopkins University School of Medicine on a Krios G3i, camera: TFS Falcon 4i, image shift at 130kx mag, exposure settings: ~4.0 s [images saved in eer format], e flux: ~8 e-/px/s with ~40 e-/Å^2 total dose, pixel size: ~0.93 Å/px (e- counting with an image dimension of 4096x4096 px), energy filter: Selectris 10 eV, defocus range: -0.8 µm to -1.8 µm (nominal).
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