EMPIAR-12914 - CryoEM dataset of M. mazei topoisomerase VI(A:E342Q)-minicircle DNA complex [26728 multi-frame micrographs composed of 1233 frames each in EER format]

EMPIAR-12914

CryoEM dataset of M. mazei topoisomerase VI(A:E342Q)-minicircle DNA complex [26728 multi-frame micrographs composed of 1233 frames each in EER format]

Publication:

Supercoiled DNA recognition and cleavage control in topoisomerase VI

Richman DE, Wendorff TJ, Rashid F, Beck C, Yan Q, Johnson HR, Eckerty RA, Fogg JM, Baker ML, Zechiedrich L, Berger JM

Nature communications 17 (2026)

PMID: 41698922

DOI: 10.1038/s41467-026-69491-0

Related EMDB entry:

EMD-49972 EMD-70206

Deposited:

2025-07-30

Released:

2026-03-04

Last modified:

2026-04-07

Imageset size:

14.07 TB

Imageset DOI:

10.6019/EMPIAR-12914

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned eer micrographs of M mazei Top6(A:E342Q)-306 bp minicircle DNA-ADPNP complex

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

26728

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(0.93, 0.93)

Details:

This dataset was collected with no stage tilt. Data were collected at the Beckman Center for CryoEM at Johns Hopkins University School of Medicine on a Krios G3i, camera: TFS Falcon 4i, image shift at 130kx mag, exposure settings: ~4.0 s [images saved in eer format], e flux: ~8 e-/px/s with ~40 e-/Å^2 total dose, pixel size: ~0.93 Å/px (e- counting with an image dimension of 4096x4096 px), energy filter: Selectris 10 eV, defocus range: -0.8 µm to -1.8 µm (nominal).

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Citations

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Yu C, Xu Z, Zeng Q, Wan X, El-Messiry H, Zhang F, Han R. (2026)

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Poudel B, Gyawali R, Dhakal A, Cheng J, Xu D. (2026)

CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

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Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

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Devarkar SC, Lomakin IB, Wang J, Grada A, Bunick CG. (2026)

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Jones HN, Deshmukh A, Pande K. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

See all citations

Protein Data Bank Japan News

[2026-06-10]

333件のPDBエントリーが新たに公開されました。（2026-06-10付）