EMPIAR-11537
Benchmark FIB SEM data (#2) of HeLa cells previously imaged by Zeiss LSM900 Airyscan microscopy [multiple data sets in TIFF format]
Publication:

CLEM-Reg: An automated point cloud based registration algorithm for correlative light and volume electron microscopy

Krentzel D, Elphick M, Domart M, Peddie CJ, Laine RF, Henriques R, Collinson LM, Jones ML

bioRxiv (2023)

Related EMDB entry:
Deposited:
2023-04-27
Released:
2023-05-22
Last modified:
2023-05-23
Imageset size:
511.90 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - single frame
  • micrographs - multiframe
1. Raw FIB SEM Run 1
Category:
micrographs - single frame
Image format:
TIFF
No. of images or tilt series:
3419
Image size:
(14976, 14976)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(50.0, 50.0)
Details:
First part of the raw data (out of 3 in total).
Images were acquired at 5 nm isotropic resolution throughout the region of interest, using a 6 us dwell time. During acquisition the SEM was operated at an accelerating voltage of 1.5 kV with 1.5 nA current.
Files:
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2. Run 2
Category:
micrographs - single frame
Image format:
TIFF
No. of images or tilt series:
156
Image size:
(14976, 14976)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(50.0, 50.0)
Details:
Second part of the raw data (out of 3 in total).
Images were acquired at 5 nm isotropic resolution throughout the region of interest, using a 6 us dwell time. During acquisition the SEM was operated at an accelerating voltage of 1.5 kV with 1.5 nA current.
Files:
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3. Run 3
Category:
micrographs - single frame
Image format:
TIFF
No. of images or tilt series:
4266
Image size:
(14976, 14976)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(50.0, 50.0)
Details:
Third part of the raw data (out of 3 in total).
Images were acquired at 5 nm isotropic resolution throughout the region of interest, using a 6 us dwell time. During acquisition the SEM was operated at an accelerating voltage of 1.5 kV with 1.5 nA current.
Files:
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4. Downscaled (20nm) aligned FIB SEM stack resliced to match the Airyscan dataset
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(3213, 1959)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(200.0, 200.0)
Details:
Processed and aligned stack of FIB SEM images downscaled to 20 nm isotropic resolution and resliced to match the orientation of the Airyscan data available on Biostudies
Files:
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5. Aligned full resolution FIB SEM stack resliced to match the Airyscan dataset
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(12850, 7835)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(50.0, 50.0)
Details:
Processed and aligned stack of FIB SEM images at full 5nm isotropic resolution and resliced to match the orientation of the Airyscan data available on Biostudies
Files:
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Files:
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