EMPIAR-11537 - Benchmark FIB SEM data (#2) of HeLa cells previously imaged by Zeiss LSM900 Airyscan microscopy [multiple data sets in TIFF format]

EMPIAR-11537

Benchmark FIB SEM data (#2) of HeLa cells previously imaged by Zeiss LSM900 Airyscan microscopy [multiple data sets in TIFF format]

Publication:

CLEM-Reg: An automated point cloud based registration algorithm for correlative light and volume electron microscopy

Krentzel D, Elphick M, Domart M, Peddie CJ, Laine RF, Henriques R, Collinson LM, Jones ML

bioRxiv (2023)

DOI: 10.1101/2023.05.11.540445

Related EMDB entry:

Deposited:

2023-04-27

Released:

2023-05-22

Last modified:

2023-05-23

Imageset size:

511.90 GB

Imageset DOI:

10.6019/EMPIAR-11537

Experimental metadata:

Download xml

Contains:

micrographs - single frame
micrographs - multiframe

Image Sets

1. Raw FIB SEM Run 1

Category:

micrographs - single frame

Image format:

TIFF

No. of images or tilt series:

3419

Image size:

(14976, 14976)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(50.0, 50.0)

Details:

First part of the raw data (out of 3 in total).
Images were acquired at 5 nm isotropic resolution throughout the region of interest, using a 6 us dwell time. During acquisition the SEM was operated at an accelerating voltage of 1.5 kV with 1.5 nA current.

Files:

2. Run 2

Category:

micrographs - single frame

Image format:

TIFF

No. of images or tilt series:

156

Image size:

(14976, 14976)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(50.0, 50.0)

Details:

Second part of the raw data (out of 3 in total).
Images were acquired at 5 nm isotropic resolution throughout the region of interest, using a 6 us dwell time. During acquisition the SEM was operated at an accelerating voltage of 1.5 kV with 1.5 nA current.

Files:

3. Run 3

Category:

micrographs - single frame

Image format:

TIFF

No. of images or tilt series:

4266

Image size:

(14976, 14976)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(50.0, 50.0)

Details:

Third part of the raw data (out of 3 in total).
Images were acquired at 5 nm isotropic resolution throughout the region of interest, using a 6 us dwell time. During acquisition the SEM was operated at an accelerating voltage of 1.5 kV with 1.5 nA current.

Files:

4. Downscaled (20nm) aligned FIB SEM stack resliced to match the Airyscan dataset

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

Image size:

(3213, 1959)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(200.0, 200.0)

Details:

Processed and aligned stack of FIB SEM images downscaled to 20 nm isotropic resolution and resliced to match the orientation of the Airyscan data available on Biostudies

Files:

5. Aligned full resolution FIB SEM stack resliced to match the Airyscan dataset

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

Image size:

(12850, 7835)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(50.0, 50.0)

Details:

Processed and aligned stack of FIB SEM images at full 5nm isotropic resolution and resliced to match the orientation of the Airyscan data available on Biostudies

Files: