FEI Titan Krios + Gatan K3, super-resolution, image shift, BioQuantum energy filter 20 eV slit, Cs correction; accelerating voltage 300 kV; dose 30 e-/px(detector)/s; dose 7.5 e-/px(image)/s; pixel size 1.08 A/px(detector); pixel size 0.54 A/px(image); exposure 2.5 s; dose/frame 1.286008 e-/A^2/frame; frame time 0.05 s; total dose 64.30041 e-/A^2; total frames 50; defocus range -1 to -2.5um; gain correction was applied on the fly during collection Picked particles star file "EsN-dhsU36mm2_particles_picked.star" ("aux_files" folder) contains all particles picked initially and is only provided for reference.
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See also the "corrected_micrographs.star" file in the "aux_files" folder. Raw micrographs were binned by 2 during motion correction. Picked particles star file "EsN-dhsU36mm2_particles_picked.star" ("aux_files" folder) contains all particles picked initially and is only provided for reference.
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non-dose-weighted micrographs from motioncor2 output
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Particle stack for map 1 after Bayesian polishing (see "EsN-dhsU36mm2_particles_map1.star" (final refinement parameters) and "EsN-dhsU36mm2_particles_shiny.star" (output from Bayesian Polishing) in "aux_files" folder)
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Particle stack for map 2 after masked subtraction (see "EsN-dhsU36mm2_particles_map2.star" (final refinement parameters) and "EsN-dhsU36mm2_particles_original_map2.star" (original particles) in "aux_files" folder)
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