EMPIAR-11521
CryoEM of E. coli RNA polymerase Sigma N bound to mismatch DNA fragment dhsU36mm2 (-12A) [multiple data sets in TIFF, MRC and MRCS formats]
Publication:

A general mechanism for transcription bubble nucleation in bacteria

Mueller AU, Chen J, Wu M, Chiu C, Nixon BT, Campbell EA, Darst SA

Proceedings of the National Academy of Sciences of the United States of America 120 (2023)

PMID: 36972428

Related EMDB entry:
Deposited:
2023-04-05
Released:
2023-05-16
Last modified:
2023-05-16
Imageset size:
8.23 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • micrographs - single frame
  • picked particles - single frame - processed
1. Unaligned micrographs (raw movies)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
7101
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.54, 0.54)
Details:
FEI Titan Krios + Gatan K3, super-resolution, image shift, BioQuantum energy filter 20 eV slit, Cs correction; accelerating voltage 300 kV; dose 30 e-/px(detector)/s; dose 7.5 e-/px(image)/s; pixel size 1.08 A/px(detector); pixel size 0.54 A/px(image); exposure 2.5 s; dose/frame 1.286008 e-/A^2/frame; frame time 0.05 s; total dose 64.30041 e-/A^2; total frames 50; defocus range -1 to -2.5um; gain correction was applied on the fly during collection
Picked particles star file "EsN-dhsU36mm2_particles_picked.star" ("aux_files" folder) contains all particles picked initially and is only provided for reference.
Files:
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2. Motion-corrected micrographs
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
7101
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.08, 1.08)
Details:
See also the "corrected_micrographs.star" file in the "aux_files" folder.
Raw micrographs were binned by 2 during motion correction.
Picked particles star file "EsN-dhsU36mm2_particles_picked.star" ("aux_files" folder) contains all particles picked initially and is only provided for reference.
Files:
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3. Non-dose-weighted motion-corrected micrographs
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
7101
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.08, 1.08)
Details:
non-dose-weighted micrographs from motioncor2 output
Files:
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4. Particle images for map 1
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
7018
Image size:
(256, 256)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.08, 1.08)
Details:
Particle stack for map 1 after Bayesian polishing (see "EsN-dhsU36mm2_particles_map1.star" (final refinement parameters) and "EsN-dhsU36mm2_particles_shiny.star" (output from Bayesian Polishing) in "aux_files" folder)
Files:
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5. Particle images for map 2 (subtracted particles)
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
398
Image size:
(256, 256)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.08, 1.08)
Details:
Particle stack for map 2 after masked subtraction (see "EsN-dhsU36mm2_particles_map2.star" (final refinement parameters) and "EsN-dhsU36mm2_particles_original_map2.star" (original particles) in "aux_files" folder)
Files:
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Files:
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