EMPIAR-11369
SBF-SEM micrographs of A. algerae spores, Ungerminated [250 micrographs in TIFF format]
Publication:

3-Dimensional organization and dynamics of the microsporidian polar tube invasion machinery

Jaroenlak P, Cammer M, Davydov A, Sall J, Usmani M, Liang F, Ekiert D, Bhabha G

PLoS pathogens 16 (2020)

PMID: 32946515

Related EMDB entry:
Deposited:
2023-01-04
Released:
2023-10-10
Last modified:
2023-10-10
Imageset size:
46.57 GB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - single frame
1. SBF-SEM micrographs of A. algerae microsporidia spores, Ungerminated
Category:
micrographs - single frame
Image format:
TIFF
No. of images or tilt series:
250
Image size:
(10000, 10000)
Pixel type:
UNSIGNED 32 BIT INTEGER
Pixel spacing:
(2.2, 2.2)
Details:
Micrographs imaged using the Gatan OnPoint BSE detector in a Zeiss Gemini 300 VP FESEM equipped with a Gatan 3View automatic microtome. The system was set to cut 50 nm slices, imaged with gas injection setting at 40% (2.9*10^-3 mBar) with Focus Charge Compensation to reduce electron accumulation charging artifacts. Images were recorded after each round of sectioning from the blockface using the SEM beam at 1.5 keV with a beam aperture size of 30 μm and a dwell time of 1.0 μsec/pixel.
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