EMPIAR-11369 - SBF-SEM micrographs of A. algerae spores, Ungerminated [250 micrographs in TIFF format]

EMPIAR-11369

SBF-SEM micrographs of A. algerae spores, Ungerminated [250 micrographs in TIFF format]

Publication:

3-Dimensional organization and dynamics of the microsporidian polar tube invasion machinery

Jaroenlak P, Cammer M, Davydov A, Sall J, Usmani M, Liang F, Ekiert D, Bhabha G

PLoS pathogens 16 (2020)

PMID: 32946515

DOI: 10.1371/journal.ppat.1008738

Related EMDB entry:

Deposited:

2023-01-04

Released:

2023-10-10

Last modified:

2023-10-10

Imageset size:

46.57 GB

Imageset DOI:

10.6019/EMPIAR-11369

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. SBF-SEM micrographs of A. algerae microsporidia spores, Ungerminated

Category:

micrographs - single frame

Image format:

TIFF

No. of images or tilt series:

250

Image size:

(10000, 10000)

Pixel type:

UNSIGNED 32 BIT INTEGER

Pixel spacing:

(2.2, 2.2)

Details:

Micrographs imaged using the Gatan OnPoint BSE detector in a Zeiss Gemini 300 VP FESEM equipped with a Gatan 3View automatic microtome. The system was set to cut 50 nm slices, imaged with gas injection setting at 40% (2.9*10^-3 mBar) with Focus Charge Compensation to reduce electron accumulation charging artifacts. Images were recorded after each round of sectioning from the blockface using the SEM beam at 1.5 keV with a beam aperture size of 30 μm and a dwell time of 1.0 μsec/pixel.

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