EMPIAR-11277
Cryo Electron Microscopy of CHMP1B/IST1 Membrane-Bound Filaments with Brominated Lipid Probes [multiple data sets in MRC format]
Publication:

Brominated lipid probes expose structural asymmetries in constricted membranes

Moss FR, Lincoff J, Tucker M, Mohammed A, Grabe M, Frost A

Nature structural & molecular biology (2023)

PMID: 36624348

Deposited:
2022-05-25
Released:
2023-01-19
Last modified:
2023-01-19
Imageset size:
3.67 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - single frame
1. Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with 0% cholesterol
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
615
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.14, 1.14)
Details:
Micrographs were acquired at 0.07 s per frame with an 8 s total exposure. Particle coordinates with refined alignments for the 17 subunit-per-turn filament are in the RELION 3.0.8-formatted star file.
Files:
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2. Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with 10% cholesterol
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
653
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.14, 1.14)
Details:
Micrographs were acquired at 0.07 s per frame with an 8 s total exposure. Particle coordinates with refined alignments for the 17 subunit-per-turn filament are in the RELION 3.0.8-formatted star file.
Files:
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3. Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with 20% cholesterol
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
816
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.14, 1.14)
Details:
Micrographs were acquired at 0.07 s per frame with an 8 s total exposure. Particle coordinates with refined alignments for the 17 subunit-per-turn filament are in the RELION 3.0.8-formatted star file.
Files:
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4. Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with 30% cholesterol
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
492
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.14, 1.14)
Details:
Micrographs were acquired at 0.07 s per frame with an 8 s total exposure. Particle coordinates with refined alignments for the 17 subunit-per-turn filament are in the RELION 3.0.8-formatted star file.
Files:
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5. Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with 40% cholesterol
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
481
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.715, 0.715)
Details:
Micrographs were acquired at 0.1 s per frame with an 11.5 s total exposure. Particle coordinates with refined alignments for the 17 subunit-per-turn filament are in the RELION 3.0.8-formatted star file.
Files:
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6. Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with lipid mixture containing POPC instead of SDPC
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
502
Image size:
(11520, 8184)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.715, 0.715)
Details:
Micrographs were acquired at 0.1 s per frame with an 11.5 s total exposure. Particle coordinates with refined alignments for the 34 subunit-per-turn filament are in the RELION 3.0.8-formatted star file.
Files:
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7. Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer without any brominated lipids
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
5684
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.835, 0.835)
Details:
Micrographs were acquired at 0.05 s per frame with an 5.9 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.
Files:
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8. Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with brominated cholesterol
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
1855
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.822, 0.822)
Details:
Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.
Files:
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9. Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with iodinated cholesterol
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
3186
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.822, 0.822)
Details:
Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.
Files:
Loading...
10. Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with brominated PIP2
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
2118
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.835, 0.835)
Details:
Micrographs were acquired at 0.05 s per frame with an 5.9 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.
Files:
Loading...
11. Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with brominated POPS
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
7806
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.822, 0.822)
Details:
Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.
Files:
Loading...
12. Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with brominated SDPC
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
2857
Image size:
(5760, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.822, 0.822)
Details:
Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.
Files:
Loading...
13. Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with 25% brominated SDPC
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
2931
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.822, 0.822)
Details:
Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.
Files:
Loading...
14. Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with 50% brominated SDPC
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
3891
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.822, 0.822)
Details:
Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.
Files:
Loading...
15. Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B(F9A/F13A)/IST1 copolymer with brominated SDPC
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
1715
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.822, 0.822)
Details:
Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.
Files:
Loading...
16. Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B(F9E/F13E)/IST1 copolymer with brominated SDPC
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
1420
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.822, 0.822)
Details:
Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.
Files:
Loading...
17. Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B(F9L/F13L)/IST1 copolymer with brominated SDPC
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
2188
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.822, 0.822)
Details:
Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.
Files:
Loading...
Files:
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