EMPIAR-11277
Cryo Electron Microscopy of CHMP1B/IST1 Membrane-Bound Filaments with Brominated Lipid Probes [multiple data sets in MRC format]
Publication:

Brominated lipid probes expose structural asymmetries in constricted membranes

Moss FR, Lincoff J, Tucker M, Mohammed A, Grabe M, Frost A

Nature structural & molecular biology (2023)

PMID: 36624348

Deposited:
2022-05-25
Released:
2023-01-19
Last modified:
2023-01-19
Imageset size:
3.67 TB
Imageset DOI:
Experimental metadata:
Contains:
  • micrographs - single frame
1. Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with 0% cholesterol
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
615
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.14, 1.14)
Details:
Micrographs were acquired at 0.07 s per frame with an 8 s total exposure. Particle coordinates with refined alignments for the 17 subunit-per-turn filament are in the RELION 3.0.8-formatted star file.
Files:
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2. Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with 10% cholesterol
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
653
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.14, 1.14)
Details:
Micrographs were acquired at 0.07 s per frame with an 8 s total exposure. Particle coordinates with refined alignments for the 17 subunit-per-turn filament are in the RELION 3.0.8-formatted star file.
Files:
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3. Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with 20% cholesterol
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
816
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.14, 1.14)
Details:
Micrographs were acquired at 0.07 s per frame with an 8 s total exposure. Particle coordinates with refined alignments for the 17 subunit-per-turn filament are in the RELION 3.0.8-formatted star file.
Files:
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4. Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with 30% cholesterol
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
492
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.14, 1.14)
Details:
Micrographs were acquired at 0.07 s per frame with an 8 s total exposure. Particle coordinates with refined alignments for the 17 subunit-per-turn filament are in the RELION 3.0.8-formatted star file.
Files:
Loading...
5. Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with 40% cholesterol
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
481
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.715, 0.715)
Details:
Micrographs were acquired at 0.1 s per frame with an 11.5 s total exposure. Particle coordinates with refined alignments for the 17 subunit-per-turn filament are in the RELION 3.0.8-formatted star file.
Files:
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6. Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with lipid mixture containing POPC instead of SDPC
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
502
Image size:
(11520, 8184)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.715, 0.715)
Details:
Micrographs were acquired at 0.1 s per frame with an 11.5 s total exposure. Particle coordinates with refined alignments for the 34 subunit-per-turn filament are in the RELION 3.0.8-formatted star file.
Files:
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7. Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer without any brominated lipids
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
5684
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.835, 0.835)
Details:
Micrographs were acquired at 0.05 s per frame with an 5.9 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.
Files:
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8. Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with brominated cholesterol
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
1855
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.822, 0.822)
Details:
Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.
Files:
Loading...
9. Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with iodinated cholesterol
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
3186
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.822, 0.822)
Details:
Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.
Files:
Loading...
10. Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with brominated PIP2
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
2118
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.835, 0.835)
Details:
Micrographs were acquired at 0.05 s per frame with an 5.9 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.
Files:
Loading...
11. Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with brominated POPS
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
7806
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.822, 0.822)
Details:
Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.
Files:
Loading...
12. Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with brominated SDPC
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
2857
Image size:
(5760, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.822, 0.822)
Details:
Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.
Files:
Loading...
13. Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with 25% brominated SDPC
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
2931
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.822, 0.822)
Details:
Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.
Files:
Loading...
14. Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B/IST1 copolymer with 50% brominated SDPC
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
3891
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.822, 0.822)
Details:
Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.
Files:
Loading...
15. Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B(F9A/F13A)/IST1 copolymer with brominated SDPC
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
1715
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.822, 0.822)
Details:
Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.
Files:
Loading...
16. Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B(F9E/F13E)/IST1 copolymer with brominated SDPC
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
1420
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.822, 0.822)
Details:
Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.
Files:
Loading...
17. Motion-corrected, dose-weighted micrographs of membrane-bound CHMP1B(F9L/F13L)/IST1 copolymer with brominated SDPC
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
2188
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.822, 0.822)
Details:
Micrographs were acquired at 0.05 s per frame with an 6.0 s total exposure. Particle coordinates with refined alignments for the 17 and 18 subunit-per-turn filaments are in the RELION 3.0.8-formatted star file.
Files:
Loading...
Files:
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Citations
Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)
Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)
Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)
Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)
See all citations
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