EMPIAR-11248 - Cryo-EM structure of non gastric H,K-ATPase alpha2 mutants [multiple data sets in TIFF format]

EMPIAR-11248

Cryo-EM structure of non gastric H,K-ATPase alpha2 mutants [multiple data sets in TIFF format]

Publication:

Structure and function of H+/K+ pump mutants reveal Na+/K+ pump mechanism

Young VC, Nakanishi H, Meyer DJ, Nishizawa T, Oshima A, Artigas P, Abe K

Nature Communications 13 (2022)

PMID: 36085139

DOI: 10.1038/s41467-022-32793-0

Related EMDB entry:

EMD-32954 EMD-32956 EMD-32957 EMD-32955

Deposited:

2022-10-03

Released:

2022-10-17

Last modified:

2022-10-17

Imageset size:

3.77 TB

Imageset DOI:

10.6019/EMPIAR-11248

Experimental metadata:

Download xml json

Deposited via PDBj

Contains:

micrographs - multiframe

Image Sets

1. K3 movies for ngHKA K794A (K+)E2-Pi state, PDB 7X21, EMD-32954

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3106

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(None, None)

Details:

- 300 kV
- Cs 2.7 mm
- 0.83 Å/pix
- 53 e-/Å2 in 50 frames

Please exclude the first frame from processing because it is corrupted due to a bug in the CDS mode.
Please use 'CountRef_abe3-1_5-015_0000_Sep08_13.42.21.mrc' as a gain reference without rotation or flipping.
Four digits (0000 to 0008) in the file name represent the beam shift directions.

Files:

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Download a list of selected files.

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rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. K3 movies for ngHKA K794S (2K+)E2-Pi state, PDB 7X22, EMD-32955

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3141

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(None, None)

Details:

- 300 kV
- Cs 2.7 mm
- 0.83 Å/pix
- 53 e-/Å2 in 50 frames

Please exclude the first frame from processing because it is corrupted due to a bug in the CDS mode.
Please use 'CountRef_abe2-1_5-A-001_0000_Feb21_16.39.37.mrc' for files under 'Movies_2-1' and 'CountRef_abe2-1_3-A-001_0000_Feb21_23.51.48.mrc' for files under 'Movies_2-3' as a gain reference without rotation or flipping.
Four digits (0000 to 0008) in the file name represent the beam shift directions.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. K3 movies for ngHKA SPWC 3Na+ꞏE1-ATP state, PDB 7X23, EMD-32956

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3627

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(None, None)

Details:

- 300 kV
- Cs 2.7 mm
- 0.83 Å/pix
- 53 e-/Å2 in 50 frames

Please exclude the first frame from processing because it is corrupted due to a bug in the CDS mode.
Please use 'CountCDSRef_abe1-1_21-A-001-1_0000_Aug02_14.03.28.mrc' as a gain reference without rotation or flipping.
Four digits (0000 to 0008) in the file name represent the beam shift directions.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

4. K3 movies for ngHKA SPWC (2K+)E2-Pi state, PDB 7X24, EMD-32957

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4150

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(None, None)

Details:

- 300 kV
- Cs 2.7 mm
- 0.83 Å/pix
- 53 e-/Å2 in 50 frames

Please exclude the first frame from processing because it is corrupted due to a bug in the CDS mode.
Please use 'CountCDSRef_abe1-1_11_0000_Jun14_15.38.45.mrc' as a gain reference without rotation or flipping.
Four digits (0000 to 0008) in the file name represent the beam shift directions.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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