EMPIAR-11248
Cryo-EM structure of non gastric H,K-ATPase alpha2 mutants [multiple data sets in TIFF format]
Publication:

Structure and function of H+/K+ pump mutants reveal Na+/K+ pump mechanism

Young VC, Nakanishi H, Meyer DJ, Nishizawa T, Oshima A, Artigas P, Abe K

Nature Communications 13 (2022)

PMID: 36085139

Related EMDB entry:
Deposited:
2022-10-03
Released:
2022-10-17
Last modified:
2022-10-17
Imageset size:
3.77 TB
Imageset DOI:
Experimental metadata:
Deposited via PDBj
Contains:
  • micrographs - multiframe
1. K3 movies for ngHKA K794A (K+)E2-Pi state, PDB 7X21, EMD-32954
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
3106
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(None, None)
Details:
- 300 kV
- Cs 2.7 mm
- 0.83 Å/pix
- 53 e-/Å2 in 50 frames

Please exclude the first frame from processing because it is corrupted due to a bug in the CDS mode.
Please use 'CountRef_abe3-1_5-015_0000_Sep08_13.42.21.mrc' as a gain reference without rotation or flipping.
Four digits (0000 to 0008) in the file name represent the beam shift directions.
Files:
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2. K3 movies for ngHKA K794S (2K+)E2-Pi state, PDB 7X22, EMD-32955
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
3141
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(None, None)
Details:
- 300 kV
- Cs 2.7 mm
- 0.83 Å/pix
- 53 e-/Å2 in 50 frames

Please exclude the first frame from processing because it is corrupted due to a bug in the CDS mode.
Please use 'CountRef_abe2-1_5-A-001_0000_Feb21_16.39.37.mrc' for files under 'Movies_2-1' and 'CountRef_abe2-1_3-A-001_0000_Feb21_23.51.48.mrc' for files under 'Movies_2-3' as a gain reference without rotation or flipping.
Four digits (0000 to 0008) in the file name represent the beam shift directions.
Files:
Loading...
3. K3 movies for ngHKA SPWC 3Na+ꞏE1-ATP state, PDB 7X23, EMD-32956
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
3627
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(None, None)
Details:
- 300 kV
- Cs 2.7 mm
- 0.83 Å/pix
- 53 e-/Å2 in 50 frames

Please exclude the first frame from processing because it is corrupted due to a bug in the CDS mode.
Please use 'CountCDSRef_abe1-1_21-A-001-1_0000_Aug02_14.03.28.mrc' as a gain reference without rotation or flipping.
Four digits (0000 to 0008) in the file name represent the beam shift directions.
Files:
Loading...
4. K3 movies for ngHKA SPWC (2K+)E2-Pi state, PDB 7X24, EMD-32957
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
4150
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(None, None)
Details:
- 300 kV
- Cs 2.7 mm
- 0.83 Å/pix
- 53 e-/Å2 in 50 frames

Please exclude the first frame from processing because it is corrupted due to a bug in the CDS mode.
Please use 'CountCDSRef_abe1-1_11_0000_Jun14_15.38.45.mrc' as a gain reference without rotation or flipping.
Four digits (0000 to 0008) in the file name represent the beam shift directions.
Files:
Loading...
Files:
Loading...
Quick Links
Citations
Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)
Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)
Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)
Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)
See all citations
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