EMPIAR-10712
Transmission electron micrographs of mouse RNF213 incubated with ATPγS [multiple data sets in TIFF format]
Publication:

E3 ubiquitin ligase RNF213 employs a non-canonical zinc finger active site and is allosterically regulated by ATP

Ahel J, Clausen T, Virdee S, Fletcher AJ, Grabarczyk DB, Deszcz L, Lehner A, Roitinger E

BiorXiv (---)

Related EMDB entry:
Deposited:
2020-06-17
Released:
2022-06-14
Last modified:
2022-06-14
Imageset size:
2.34 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. unaligned multi-frame micrographs from a dataset collected without grid tilting
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
5950
Image size:
(3838, 3710)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(1.04, 1.04)
Details:
set1_noTilt/raw_micrographs/ == unaligned multi-frame micrographs, not gain-corrected
==
set1_noTilt/set1_GainReference.mrc == gain reference for the dataset
set1_noTilt/set1_micrographs_ctf.star == relion 3.1 star file describing the gain-corrected dose-weighed motion-corrected micrographs
set1_noTilt/micrographs_ctf_motioncorr/ == gain-corrected dose-weighed motion-corrected micrographs
==
set1_noTilt/coordinates_initial_cryolo/ == particle coordinates picked by cryolo
set1_noTilt/coordinates_final_refined/ == particle coordinates for the particles used in the final reconstruction
set1_noTilt/particles/ == particles used in the final reconstruction, Bayesian-polished by relion 3.1
==
./refined_particles_combined.star == relion 3.1 star file describing the particles after the final reconstruction, including angular assignment
==
./cryolo_YOLO_RNF213.h5 == cryolo network model used for initial particles picking
./cryolo_YOLO_RNF213_config.json == config file for the cryolo network model used for initial particles picking
./MTF_K2summit_300kV.star == MTF file used for MTF correction for this dataset
Files:
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2. unaligned multi-frame micrographs from a dataset collected with a 30 deg grid tilt
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
3055
Image size:
(5760, 4092)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(1.052, 1.052)
Details:
set2_withTilt/raw_micrographs/ == unaligned multi-frame micrographs, not gain-corrected
==
set2_withTilt/set2_GainReference.mrc == gain reference for the dataset
set2_withTilt/set2_micrographs_ctf.star == relion 3.1 star file describing the gain-corrected dose-weighed motion-corrected micrographs
set2_withTilt/micrographs_ctf_motioncorr/ == gain-corrected dose-weighed motion-corrected micrographs
==
set2_withTilt/coordinates_initial_3Dpick/ == particle coordinates picked by the relion 3D template picker
set2_withTilt/coordinates_final_refined/ == particle coordinates for the particles used in the final reconstruction
set2_withTilt/particles/ == particles used in the final reconstruction, Bayesian-polished by relion 3.1
==
./refined_particles_combined.star == relion 3.1 star file describing the particles after the final reconstruction, including angular assignment
==
./mtf_k3_standard_300kV_FL2.star == MTF file used for MTF correction for this dataset
Files:
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Files:
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