EMPIAR-10712
Transmission electron micrographs of mouse RNF213 incubated with ATPγS [multiple data sets in TIFF format]
Publication:

E3 ubiquitin ligase RNF213 employs a non-canonical zinc finger active site and is allosterically regulated by ATP

Ahel J, Clausen T, Virdee S, Fletcher AJ, Grabarczyk DB, Deszcz L, Lehner A, Roitinger E

BiorXiv (---)

Related EMDB entry:
Deposited:
2020-06-17
Released:
2022-06-14
Last modified:
2022-06-14
Imageset size:
2.34 TB
Imageset DOI:
Experimental metadata:
Contains:
  • micrographs - multiframe
1. unaligned multi-frame micrographs from a dataset collected without grid tilting
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
5950
Image size:
(3838, 3710)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(1.04, 1.04)
Details:
set1_noTilt/raw_micrographs/ == unaligned multi-frame micrographs, not gain-corrected
==
set1_noTilt/set1_GainReference.mrc == gain reference for the dataset
set1_noTilt/set1_micrographs_ctf.star == relion 3.1 star file describing the gain-corrected dose-weighed motion-corrected micrographs
set1_noTilt/micrographs_ctf_motioncorr/ == gain-corrected dose-weighed motion-corrected micrographs
==
set1_noTilt/coordinates_initial_cryolo/ == particle coordinates picked by cryolo
set1_noTilt/coordinates_final_refined/ == particle coordinates for the particles used in the final reconstruction
set1_noTilt/particles/ == particles used in the final reconstruction, Bayesian-polished by relion 3.1
==
./refined_particles_combined.star == relion 3.1 star file describing the particles after the final reconstruction, including angular assignment
==
./cryolo_YOLO_RNF213.h5 == cryolo network model used for initial particles picking
./cryolo_YOLO_RNF213_config.json == config file for the cryolo network model used for initial particles picking
./MTF_K2summit_300kV.star == MTF file used for MTF correction for this dataset
Files:
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2. unaligned multi-frame micrographs from a dataset collected with a 30 deg grid tilt
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
3055
Image size:
(5760, 4092)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(1.052, 1.052)
Details:
set2_withTilt/raw_micrographs/ == unaligned multi-frame micrographs, not gain-corrected
==
set2_withTilt/set2_GainReference.mrc == gain reference for the dataset
set2_withTilt/set2_micrographs_ctf.star == relion 3.1 star file describing the gain-corrected dose-weighed motion-corrected micrographs
set2_withTilt/micrographs_ctf_motioncorr/ == gain-corrected dose-weighed motion-corrected micrographs
==
set2_withTilt/coordinates_initial_3Dpick/ == particle coordinates picked by the relion 3D template picker
set2_withTilt/coordinates_final_refined/ == particle coordinates for the particles used in the final reconstruction
set2_withTilt/particles/ == particles used in the final reconstruction, Bayesian-polished by relion 3.1
==
./refined_particles_combined.star == relion 3.1 star file describing the particles after the final reconstruction, including angular assignment
==
./mtf_k3_standard_300kV_FL2.star == MTF file used for MTF correction for this dataset
Files:
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Files:
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Citations
Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)
Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)
Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)
Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)
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