EMPIAR-13079 - Cryo-electron microscopy of LetAB from Escherichia coli [multiple data sets in TIFF format]

EMPIAR-13079

Cryo-electron microscopy of LetAB from Escherichia coli [multiple data sets in TIFF format]

Publication:

LetA defines a structurally distinct transporter family involved in lipid trafficking

Santarossa CC, Li Y, Yousef S, Hasdemir HS, Rodriguez CC, Haase MB, Baek M, Coudray N, Pavek JG, Focke KN, Silverberg AL, Bautista C, Yeh J, Marty MT, Baker D, Tajkhorshid E, Ekiert DC, Bhabha G

bioRxiv (2025)

PMID: 40166208

DOI: 10.1101/2025.03.21.644421

Related EMDB entry:

EMD-49152 EMD-49149 EMD-49151 EMD-49150

Deposited:

2025-10-26

Released:

2025-11-20

Last modified:

2025-11-20

Imageset size:

6.23 TB

Imageset DOI:

10.6019/EMPIAR-13079

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of E. coli LetAB

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

5372

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.5413, 0.5413)

Details:

TIFF files correspond to raw movie frames collected at no tilt with a total electron dose of 51 e–/Å^2 over a 2 second exposure time. Frames were collected in Leginon using super-resolution mode (super-resolution pixel size of 0.5413 Å/pix).

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2. Unaligned, tilted (-30 degree) multi-frame micrographs of E. coli LetAB

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

7083

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.5413, 0.5413)

Details:

TIFF files correspond to raw movie frames collected at a -30 degree tilt with a total electron dose of 51 e–/Å^2 over a 2 second exposure time. Frames were collected in Leginon using super-resolution mode (super-resolution pixel size of 0.5413 Å/pix).

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

GTpick: A deep neural network for Cryo-EM particle detection.

Ni S, Yang C, Liu Y, Zhang Y, Shi Y, Qian A, Kong R, Chang S. (2025)

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

DiameTR: A cryo-EM tool for diameter sorting of tubular samples.

Peng R, Elkhaligy H, Grant T, Stagg SM. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Localization of macromolecules in crowded cellular cryo-electron tomograms from extremely sparse labels.

Uddin MR, Ahmed AY, Tabib HMS, Tahmid MT, Alam MZU, Freyberg Z, Xu M. (2025)

Structural insight into bicarbonate-mediated carboxylation by human vitamin K-dependent carboxylase.

Wu K, Wang Z, Yao D, Li S, Wang X, Zhang Y, Cao M, Shen Y, Xing S, Wu J, Lei M, Lan P. (2025)

Conserved hydrophilic checkpoints tune FocA-mediated formate:H<sup>+</sup> symport.

Tüting C, Janson K, Kammel M, Ihling C, Lorenz J, Kyrilis FL, Hamdi F, Erdmann C, Sinz A, Sawers RG, Kastritis PL. (2025)

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