EMPIAR-12904
CryoEM dataset of M. mazei topoisomerase VI-minicircle DNA complex [50906 multi-frame micrographs composed of 0 frames each in EER format]
Publication:

Supercoiled DNA recognition and cleavage control in topoisomerase VI

Richman DE, Wendorff TJ, Rashid F, Beck C, Yan Q, Johnson HR, Eckerty RA, Fogg JM, Baker ML, Zechiedrich L, Berger JM

Nature communications 17 (2026)

PMID: 41698922

Related EMDB entry:
Deposited:
2025-07-30
Released:
2026-03-02
Last modified:
2026-04-07
Imageset size:
29.64 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. Unaligned eer micrographs of WT M mazei Top6-327 bp minicircle DNA-ADPNP complex
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
50906
Image size:
(4096, 4096)
Pixel type:
BIT
Pixel spacing:
(0.959, 0.959)
Details:
~80% of this dataset was collected at 30 degree stage tilt (n23feb, may, jun, jul folders). The n23jan folder was collected at 0 degree tilt. Data were collected at National Center for CryoEM Access and Training (New York Structural Biology Center) on Krios 7 "Matthew", camera: TFS Falcon IV, image shift at 130kx mag, exposure settings: 7.0 s [images saved in eer format], e flux: ~6 e-/px/s with ~50 e-/Å total dose, pixel size: ~0.959Å/px (nominal) [0.926Å(calibrated against apoF)] (e- counting with an image dimension of 4096x4096 px), energy filter: Selectris 10-20 eV, defocus range: -0.8 µm to -2.5 µm (nominal).
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