EMPIAR-12904 - CryoEM dataset of M. mazei topoisomerase VI-minicircle DNA complex [50906 multi-frame micrographs composed of 0 frames each in EER format]

EMPIAR-12904

CryoEM dataset of M. mazei topoisomerase VI-minicircle DNA complex [50906 multi-frame micrographs composed of 0 frames each in EER format]

Publication:

Supercoiled DNA recognition and cleavage control in topoisomerase VI

Richman DE, Wendorff TJ, Rashid F, Beck C, Yan Q, Johnson HR, Eckerty RA, Fogg JM, Baker ML, Zechiedrich L, Berger JM

Nature communications 17 (2026)

PMID: 41698922

DOI: 10.1038/s41467-026-69491-0

Related EMDB entry:

EMD-70239 EMD-70232 EMD-70259

Deposited:

2025-07-30

Released:

2026-03-02

Last modified:

2026-04-07

Imageset size:

29.64 TB

Imageset DOI:

10.6019/EMPIAR-12904

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned eer micrographs of WT M mazei Top6-327 bp minicircle DNA-ADPNP complex

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

50906

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(0.959, 0.959)

Details:

~80% of this dataset was collected at 30 degree stage tilt (n23feb, may, jun, jul folders). The n23jan folder was collected at 0 degree tilt. Data were collected at National Center for CryoEM Access and Training (New York Structural Biology Center) on Krios 7 "Matthew", camera: TFS Falcon IV, image shift at 130kx mag, exposure settings: 7.0 s [images saved in eer format], e flux: ~6 e-/px/s with ~50 e-/Å total dose, pixel size: ~0.959Å/px (nominal) [0.926Å(calibrated against apoF)] (e- counting with an image dimension of 4096x4096 px), energy filter: Selectris 10-20 eV, defocus range: -0.8 µm to -2.5 µm (nominal).

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Citations

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Protein Data Bank Japan News

[2026-06-17]

324件のPDBエントリーが新たに公開されました。（2026-06-17付）