EMPIAR-11368
SBF-SEM micrographs of A. algerae microsporidia spores, 45 min germination [300 micrographs in TIFF format]
Publication:

Energetics of the Microsporidian Polar Tube Invasion Machinery

Chang R, Davydov A, Jaroenlak P, Budaitis B, Ekiert D, Bhabha G, Prakash M

ELife (2023)

Related EMDB entry:
Deposited:
2023-01-06
Released:
2023-10-13
Last modified:
2023-10-13
Imageset size:
55.88 GB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - single frame
1. SBF-SEM micrographs of A. algerae microsporidia spores, 45 min germination
Category:
micrographs - single frame
Image format:
TIFF
No. of images or tilt series:
300
Image size:
(10000, 10000)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(2.2, 2.2)
Details:
Micrographs imaged using the Gatan OnPoint BSE detector in a Zeiss Gemini 300 VP FESEM equipped with a Gatan 3View automatic microtome. The system was set to cut 40 nm slices, imaged with gas injection setting at 40% (2.9*10^-3 mBar) with Focus Charge Compensation to reduce electron accumulation charging artifacts. Images were recorded after each round of sectioning from the blockface using the SEM beam at 1.5 keV with a beam aperture size of 30 μm and a dwell time of 2.0 μsec/pixel.
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