EMPIAR-11364
Cryogenic electron tomographs of Coxsackievirus A9 treated with endosomal ionic condition buffer [1964 multi-frame micrographs composed of 30 frames each in MRC format]
Publication:

Structural Studies Reveal that Endosomal Cations Promote Formation of Infectious Coxsackievirus A9 A-Particles, Facilitating RNA and VP4 Release

Domanska A, Plavec Z, Ruokolainen V, Löflund B, Marjomäki V, Butcher SJ

Journal of virology 96 (2022)

PMID: 36448797

Related EMDB entry:
Deposited:
2022-12-12
Released:
2023-01-20
Last modified:
2023-01-20
Imageset size:
1.80 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
1. Raw micrographs of CVA9 treated with ionic buffer condtions 3h
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
1964
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(1.24, 1.24)
Details:
Micrographs were collected on FEI TAlos Arctica operating at 200 kV with a Falcon III direct electron detector at a nominal magnification of ×120,000. Each movie consisted of 30 frames with an exposure time of 1 s per frame. The total electron dose was approximately 30 electrons per Å2.
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Citations

Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)
Rigden DJ, Fernández XM. (2023)
Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)
Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)
Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)