EMPIAR-11364
Cryogenic electron tomographs of Coxsackievirus A9 treated with endosomal ionic condition buffer [1964 multi-frame micrographs composed of 30 frames each in MRC format]
Publication:

Structural Studies Reveal that Endosomal Cations Promote Formation of Infectious Coxsackievirus A9 A-Particles, Facilitating RNA and VP4 Release

Domanska A, Plavec Z, Ruokolainen V, Löflund B, Marjomäki V, Butcher SJ

Journal of virology 96 (2022)

PMID: 36448797

Related EMDB entry:
Deposited:
2022-12-12
Released:
2023-01-20
Last modified:
2023-01-20
Imageset size:
1.80 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
1. Raw micrographs of CVA9 treated with ionic buffer condtions 3h
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
1964
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(1.24, 1.24)
Details:
Micrographs were collected on FEI TAlos Arctica operating at 200 kV with a Falcon III direct electron detector at a nominal magnification of ×120,000. Each movie consisted of 30 frames with an exposure time of 1 s per frame. The total electron dose was approximately 30 electrons per Å2.
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