EMPIAR-11299
Reconstituted USP1, UAF1, FANCI, mono-ubiquitinated FANCD2 and the inhibitor ML323 [10998 multi-frame micrographs composed of 40 frames each in TIFF format]
Publication:

Cryo-EM reveals a mechanism of USP1 inhibition through a cryptic binding site

Rennie ML, Arkinson C, Chaugule VK, Walden H

Science advances 8 (2022) eabq6353-N/A

PMID: 36170365

Deposited:
2022-11-04
Released:
2022-11-15
Last modified:
2022-11-15
Imageset size:
2.32 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
1. Unaligned multi-frame micrographs of reconstituted USP1, UAF1, FANCI, monoubiquitinated FANCD2, DNA, and ML323
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
10998
Image size:
(4092, 5760)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.06, 1.06)
Details:
Each multi-frame raw micrograph is composed of 40 frames each in compressed TIFF format, along with a separate gain reference. Multi-frame micrographs were collected at 81,000x nominal magnification at 15.46 e-/pix/s in super-resolution mode with 2x binning on a Gatan K3. Data collected on KriosIV at eBIC on 2021-11-29.
Sample contained 9.3 μM USP1, 9.3 μM UAF1, 1.8 μM FANCI, 1.8 μM FANCD2 monoubiquitinated on K561, 2.2 μM dsDNA (61 base pairs), and 18.3 μM ML323.
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