EMPIAR-11110 - Cryo-EM structure of Cas13bt3–guide RNA–target RNA complex [2772 multi-frame micrographs composed of 48 frames each in TIFF format]

EMPIAR-11110

Cryo-EM structure of Cas13bt3–guide RNA–target RNA complex [2772 multi-frame micrographs composed of 48 frames each in TIFF format]

Publication:

Structure and engineering of the minimal type VI CRISPR-Cas13bt3

Nakagawa R, Kannan S, Altae-Tran H, Takeda SN, Tomita A, Hirano H, Kusakizako T, Nishizawa T, Yamashita K, Zhang F, Nishimasu H, Nureki O

Molecular cell 82 (2022) 3178-3192.e5

PMID: 36027912

DOI: 10.1016/j.molcel.2022.08.001

Related EMDB entry:

EMD-32118

Deposited:

2022-06-28

Released:

2022-09-20

Last modified:

2022-09-20

Imageset size:

616.89 GB

Imageset DOI:

10.6019/EMPIAR-11110

Experimental metadata:

Download xml json

Deposited via PDBj

Contains:

micrographs - multiframe

Image Sets

1. Unaligned movies for Cas13bt3–guide RNA–target RNA complex

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2772

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

HT: 300 kV
Cs: 2.7 mm
Flux: 1 e/Å2/frame
Please use 'CountRef_nakagawa4_80-1_0000_Jun01_11.35.15.mrc' as a gain reference without rotation or flipping.
The calibrated pixel size is 0.83 Å, while the nominal pixel size of 0.85 Å is shown in the tiff header.

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

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