HT: 300 kV Cs: 2.7 mm Flux: 1 e/Å2/frame Please use 'CountRef_nakagawa4_80-1_0000_Jun01_11.35.15.mrc' as a gain reference without rotation or flipping. The calibrated pixel size is 0.83 Å, while the nominal pixel size of 0.85 Å is shown in the tiff header.