EMPIAR-11110 - Cryo-EM structure of Cas13bt3–guide RNA–target RNA complex [2772 multi-frame micrographs composed of 48 frames each in TIFF format]

EMPIAR-11110

Cryo-EM structure of Cas13bt3–guide RNA–target RNA complex [2772 multi-frame micrographs composed of 48 frames each in TIFF format]

Publication:

Structure and engineering of the minimal type VI CRISPR-Cas13bt3

Nakagawa R, Kannan S, Altae-Tran H, Takeda SN, Tomita A, Hirano H, Kusakizako T, Nishizawa T, Yamashita K, Zhang F, Nishimasu H, Nureki O

Molecular cell 82 (2022) 3178-3192.e5

PMID: 36027912

DOI: 10.1016/j.molcel.2022.08.001

Related EMDB entry:

EMD-32118

Deposited:

2022-06-28

Released:

2022-09-20

Last modified:

2022-09-20

Imageset size:

616.89 GB

Imageset DOI:

10.6019/EMPIAR-11110

Experimental metadata:

Download xml json

Deposited via PDBj

Contains:

micrographs - multiframe

Image Sets

1. Unaligned movies for Cas13bt3–guide RNA–target RNA complex

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2772

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

HT: 300 kV
Cs: 2.7 mm
Flux: 1 e/Å2/frame
Please use 'CountRef_nakagawa4_80-1_0000_Jun01_11.35.15.mrc' as a gain reference without rotation or flipping.
The calibrated pixel size is 0.83 Å, while the nominal pixel size of 0.85 Å is shown in the tiff header.

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Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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Citations

Structural basis for HIV-1 antagonism of host APOBEC3G via Cullin E3 ligase.

Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)

The 2023 Nucleic Acids Research Database Issue and the online molecular biology database collection.

Rigden DJ, Fernández XM. (2023)

EMPIAR: the Electron Microscopy Public Image Archive.

Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)

CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM.

Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)

Structure of a RecT/Redβ family recombinase in complex with a duplex intermediate of DNA annealing.

Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)

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