EMPIAR-11110 - Cryo-EM structure of Cas13bt3–guide RNA–target RNA complex [2772 multi-frame micrographs composed of 48 frames each in TIFF format]

EMPIAR-11110

Cryo-EM structure of Cas13bt3–guide RNA–target RNA complex [2772 multi-frame micrographs composed of 48 frames each in TIFF format]

Publication:

Structure and engineering of the minimal type VI CRISPR-Cas13bt3

Nakagawa R, Kannan S, Altae-Tran H, Takeda SN, Tomita A, Hirano H, Kusakizako T, Nishizawa T, Yamashita K, Zhang F, Nishimasu H, Nureki O

Molecular cell 82 (2022) 3178-3192.e5

PMID: 36027912

DOI: 10.1016/j.molcel.2022.08.001

Related EMDB entry:

EMD-32118

Deposited:

2022-06-28

Released:

2022-09-20

Last modified:

2022-09-20

Imageset size:

616.89 GB

Imageset DOI:

10.6019/EMPIAR-11110

Experimental metadata:

Download xml json

Deposited via PDBj

Contains:

micrographs - multiframe

Image Sets

1. Unaligned movies for Cas13bt3–guide RNA–target RNA complex

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2772

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

HT: 300 kV
Cs: 2.7 mm
Flux: 1 e/Å2/frame
Please use 'CountRef_nakagawa4_80-1_0000_Jun01_11.35.15.mrc' as a gain reference without rotation or flipping.
The calibrated pixel size is 0.83 Å, while the nominal pixel size of 0.85 Å is shown in the tiff header.

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Citations

AlignPCA-2D: PCA-reduced Euclidean vector alignment for 2D classification in cryo-EM.

Ramírez-Aportela E, Zarrabeitia OL, Fonseca YC, Ceska T, Subramaniam S, Carazo JM, Sorzano COS. (2026)

A variational framework with composite sparse regularization for cryo-electron tomography reconstruction.

Yu C, Xu Z, Zeng Q, Wan X, El-Messiry H, Zhang F, Han R. (2026)

Helical reconstruction of amyloids in cryoSPARC.

Schaefer JH, O'Neill RT, Donnelly JP, Powers ET, Kelly JW, Lander GC. (2026)

An unattended image-processing pipeline for on-the-fly quality assessment and 3D exploration in cryo-EM.

Marchán Torres D, Conesa P, Garcia A, Iceta M, Broche L, Gragera M, Linares R, Kwong HS, Chichón FJ, Svensson O, Arranz R, Effantin G, Kandiah E, Carazo JM, Sorzano COS. (2026)

CryoFSL: an annotation-efficient, few-shot learning framework for robust protein particle picking in cryo-electron microscopy micrographs.

Poudel B, Gyawali R, Dhakal A, Cheng J, Xu D. (2026)

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

Unbend, correction of local beam-induced sample motion in cryo-EM images using a 3D spline model.

Kong L, Zottig X, Elferich J, Grigorieff N. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

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