EMPIAR-11110 - Cryo-EM structure of Cas13bt3–guide RNA–target RNA complex [2772 multi-frame micrographs composed of 48 frames each in TIFF format]

EMPIAR-11110

Cryo-EM structure of Cas13bt3–guide RNA–target RNA complex [2772 multi-frame micrographs composed of 48 frames each in TIFF format]

Publication:

Structure and engineering of the minimal type VI CRISPR-Cas13bt3

Nakagawa R, Kannan S, Altae-Tran H, Takeda SN, Tomita A, Hirano H, Kusakizako T, Nishizawa T, Yamashita K, Zhang F, Nishimasu H, Nureki O

Molecular cell 82 (2022) 3178-3192.e5

PMID: 36027912

DOI: 10.1016/j.molcel.2022.08.001

Related EMDB entry:

EMD-32118

Deposited:

2022-06-28

Released:

2022-09-20

Last modified:

2022-09-20

Imageset size:

616.89 GB

Imageset DOI:

10.6019/EMPIAR-11110

Experimental metadata:

Download xml json

Deposited via PDBj

Contains:

micrographs - multiframe

Image Sets

1. Unaligned movies for Cas13bt3–guide RNA–target RNA complex

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2772

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

HT: 300 kV
Cs: 2.7 mm
Flux: 1 e/Å2/frame
Please use 'CountRef_nakagawa4_80-1_0000_Jun01_11.35.15.mrc' as a gain reference without rotation or flipping.
The calibrated pixel size is 0.83 Å, while the nominal pixel size of 0.85 Å is shown in the tiff header.

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Citations

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