EMPIAR-10980 - Cryo-EM structures of monomeric and dimeric human somatic angiotensin I-converting enzyme (apo form) [7689 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-10980

Cryo-EM structures of monomeric and dimeric human somatic angiotensin I-converting enzyme (apo form) [7689 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Cryo-EM reveals mechanisms of angiotensin I-converting enzyme allostery and dimerization

Lubbe L, Sewell BT, Woodward JD, Sturrock ED

The EMBO journal (2022)

PMID: 35818993

DOI: 10.15252/embj.2021110550

Related EMDB entry:

EMD-13801 EMD-13797 EMD-13804 EMD-13803 EMD-13799 EMD-13802

Deposited:

2022-03-08

Released:

2022-07-18

Last modified:

2022-07-18

Imageset size:

3.85 TB

Imageset DOI:

10.6019/EMPIAR-10980

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame cryo-EM micrographs of human somatic angiotensin I-converting enzyme in the apo state

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

7689

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.53, 0.53)

Details:

The 7689 raw unaligned micrograph movies are found in two subdirectories and each movie is composed of 40 movie frames (accumulating 43 electron per squared Angstrom in a 3 seconds exposure). Data was collected in super-resolution mode at 0.53 Angstrom per pixel (Cs: 2.7mm; Acceleration voltage: 300kV; Magnification: 81000x; Defocus: -1.8 to -3 micrometer). The gain.mrc file is included and should be applied (flip the gain image upside down).

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