EMPIAR-10980
Cryo-EM structures of monomeric and dimeric human somatic angiotensin I-converting enzyme (apo form) [7689 multi-frame micrographs composed of 40 frames each in TIFF format]
Publication:

Cryo-EM reveals mechanisms of angiotensin I-converting enzyme allostery and dimerization

Lubbe L, Sewell BT, Woodward JD, Sturrock ED

The EMBO journal (2022)

PMID: 35818993

Deposited:
2022-03-08
Released:
2022-07-18
Last modified:
2022-07-18
Imageset size:
3.85 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
1. Unaligned multi-frame cryo-EM micrographs of human somatic angiotensin I-converting enzyme in the apo state
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
7689
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.53, 0.53)
Details:
The 7689 raw unaligned micrograph movies are found in two subdirectories and each movie is composed of 40 movie frames (accumulating 43 electron per squared Angstrom in a 3 seconds exposure). Data was collected in super-resolution mode at 0.53 Angstrom per pixel (Cs: 2.7mm; Acceleration voltage: 300kV; Magnification: 81000x; Defocus: -1.8 to -3 micrometer). The gain.mrc file is included and should be applied (flip the gain image upside down).
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