EMPIAR-10847 - Cryo-FIB-SEM volume in a Sum159 human cell line [20 micrographs in TIFF format]

EMPIAR-10847

Cryo-FIB-SEM volume in a Sum159 human cell line [20 micrographs in TIFF format]

Publication:

A modular platform for automated cryo-FIB workflows

Klumpe S, Fung HKH, Goetz SK, Zagoriy I, Hampoelz B, Zhang X, Erdmann PS, Baumbach J, Müller CW, Beck M, Plitzko JM, Mahamid J

eLife 10 (2021)

PMID: 34951584

DOI: 10.7554/elife.70506

Related EMDB entry:

Deposited:

2021-08-18

Released:

2022-01-12

Last modified:

2022-01-12

Imageset size:

120.13 MB

Imageset DOI:

10.6019/EMPIAR-10847

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Unaligned FIB-SEM slices of a Sum159 cell

Category:

micrographs - single frame

Image format:

TIFF

No. of images or tilt series:

Image size:

(3072, 2048)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(103.77, 103.77)

Details:

FIB-SEM performed on a TF Aquilos. SEM images were recorded at 5 kV, 50 pA, with a pixel dwell time of 1 μs and line integration of 16, using an Everhart-Thornley detector. FIB milling was performed in 100 nm-steps at a 52-degree angle relative to the electron beam.

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