EMPIAR-10847 - Cryo-FIB-SEM volume in a Sum159 human cell line [20 micrographs in TIFF format]

EMPIAR-10847

Cryo-FIB-SEM volume in a Sum159 human cell line [20 micrographs in TIFF format]

Publication:

A modular platform for automated cryo-FIB workflows

Klumpe S, Fung HKH, Goetz SK, Zagoriy I, Hampoelz B, Zhang X, Erdmann PS, Baumbach J, Müller CW, Beck M, Plitzko JM, Mahamid J

eLife 10 (2021)

PMID: 34951584

DOI: 10.7554/elife.70506

Related EMDB entry:

Deposited:

2021-08-18

Released:

2022-01-12

Last modified:

2022-01-12

Imageset size:

120.13 MB

Imageset DOI:

10.6019/EMPIAR-10847

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Unaligned FIB-SEM slices of a Sum159 cell

Category:

micrographs - single frame

Image format:

TIFF

No. of images or tilt series:

Image size:

(3072, 2048)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(103.77, 103.77)

Details:

FIB-SEM performed on a TF Aquilos. SEM images were recorded at 5 kV, 50 pA, with a pixel dwell time of 1 μs and line integration of 16, using an Everhart-Thornley detector. FIB milling was performed in 100 nm-steps at a 52-degree angle relative to the electron beam.

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Citations

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

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Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Jones HN, Deshmukh A, Pande K. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

Point cloud deformation modeling for particle selection following cryo-EM 2D classification.

Wang X, Mo Z, Li F, Zhang F, Wan X. (2026)

ICECREAM: high-fidelity equivariant cryo-electron tomography.

Kishore V, Debarnot V, Righetto RD, Engel BD, Dokmanić I. (2026)

CryoSift: an accessible and automated CNN-driven tool for cryo-EM 2D class selection.

Schäfer JH, Calza A, Hom K, Damodar P, Peng R, Bogdanović N, Lander GC, Stagg SM, Cianfrocco MA. (2025)