EMPIAR-10847
Cryo-FIB-SEM volume in a Sum159 human cell line [20 micrographs in TIFF format]
Publication:

A modular platform for automated cryo-FIB workflows

Klumpe S, Fung HKH, Goetz SK, Zagoriy I, Hampoelz B, Zhang X, Erdmann PS, Baumbach J, Müller CW, Beck M, Plitzko JM, Mahamid J

eLife 10 (2021)

PMID: 34951584

Related EMDB entry:
Deposited:
2021-08-18
Released:
2022-01-12
Last modified:
2022-01-12
Imageset size:
120.13 MB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - single frame
1. Unaligned FIB-SEM slices of a Sum159 cell
Category:
micrographs - single frame
Image format:
TIFF
No. of images or tilt series:
20
Image size:
(3072, 2048)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(103.77, 103.77)
Details:
FIB-SEM performed on a TF Aquilos. SEM images were recorded at 5 kV, 50 pA, with a pixel dwell time of 1 μs and line integration of 16, using an Everhart-Thornley detector. FIB milling was performed in 100 nm-steps at a 52-degree angle relative to the electron beam.
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