EMPIAR-10595
Human NLRP1-DPP9-VbP complex [multiple data sets in TIFF format]
Publication:

DPP9 sequesters the C terminus of NLRP1 to repress inflammasome activation

Hollingsworth LR, Sharif H, Griswold AR, Fontana P, Mintseris J, Dagbay KB, Paulo JA, Gygi SP, Bachovchin DA, Wu H

Nature (2021)

PMID: 33731932

Related EMDB entry:
Deposited:
2020-12-15
Released:
2021-04-14
Last modified:
2021-04-14
Imageset size:
1.18 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - focal pairs - unprocessed
  • micrographs - multiframe
1. Unaligned multi frame micographs of NLRP1-DPP9-VbP-noTILT
Category:
micrographs - focal pairs - unprocessed
Image format:
TIFF
No. of images or tilt series:
3553
Image size:
(None, None)
Pixel type:
BIT
Pixel spacing:
(0.825, 0.825)
Details:
The defocus range between −0.8 to −2.2 μm and to record two shots for each of the four holes per stage movement through image shift. All movies were exposed with a total dose of 52.0 e-/Å2 for 2.0 s fractionated over 50 frames.
Files:
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2. Unaligned multi frame micographs of NLRP1-DPP9-VbP-TILT
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1954
Image size:
(None, None)
Pixel type:
BIT
Pixel spacing:
(0.825, 0.825)
Details:
An ideal tilt angle of 37° was calculated from this processed dataset using cryoEF. The defocus range between −1.5 to −3.0 μm with two shots recorded for each hole per stage movement through image shift. All movies were
exposed with a total dose of 65.0 e-/Å2 for 2.4 s fractionated over 43 frames
Files:
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Files:
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