EMPIAR-10595 - Human NLRP1-DPP9-VbP complex [multiple data sets in TIFF format]

EMPIAR-10595

Human NLRP1-DPP9-VbP complex [multiple data sets in TIFF format]

Publication:

DPP9 sequesters the C terminus of NLRP1 to repress inflammasome activation

Hollingsworth LR, Sharif H, Griswold AR, Fontana P, Mintseris J, Dagbay KB, Paulo JA, Gygi SP, Bachovchin DA, Wu H

Nature (2021)

PMID: 33731932

DOI: 10.1038/s41586-021-03350-4

Related EMDB entry:

EMD-22075

Deposited:

2020-12-15

Released:

2021-04-14

Last modified:

2021-04-14

Imageset size:

1.18 TB

Imageset DOI:

10.6019/EMPIAR-10595

Experimental metadata:

Download xml json

Contains:

micrographs - focal pairs - unprocessed
micrographs - multiframe

Image Sets

1. Unaligned multi frame micographs of NLRP1-DPP9-VbP-noTILT

Category:

micrographs - focal pairs - unprocessed

Image format:

TIFF

No. of images or tilt series:

3553

Image size:

(None, None)

Pixel type:

BIT

Pixel spacing:

(0.825, 0.825)

Details:

The defocus range between −0.8 to −2.2 μm and to record two shots for each of the four holes per stage movement through image shift. All movies were exposed with a total dose of 52.0 e-/Å2 for 2.0 s fractionated over 50 frames.

Files:

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選択したファイルのリストをダウンロードします。

rsyncコマンド等でファイルリストを指定してダウンロードすることが可能です。

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Unaligned multi frame micographs of NLRP1-DPP9-VbP-TILT

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1954

Image size:

(None, None)

Pixel type:

BIT

Pixel spacing:

(0.825, 0.825)

Details:

An ideal tilt angle of 37° was calculated from this processed dataset using cryoEF. The defocus range between −1.5 to −3.0 μm with two shots recorded for each hole per stage movement through image shift. All movies were
exposed with a total dose of 65.0 e-/Å2 for 2.4 s fractionated over 43 frames

Files:

選択したファイルを無圧縮zipファイルにアーカイブ化してダウンロードします。

rsync、aspera、globus 等の利用をおすすめします。

選択したファイルのリストをダウンロードします。

rsyncコマンド等でファイルリストを指定してダウンロードすることが可能です。

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

A variational framework with composite sparse regularization for cryo-electron tomography reconstruction.

Yu C, Xu Z, Zeng Q, Wan X, El-Messiry H, Zhang F, Han R. (2026)

CryoFSL: an annotation-efficient, few-shot learning framework for robust protein particle picking in cryo-electron microscopy micrographs.

Poudel B, Gyawali R, Dhakal A, Cheng J, Xu D. (2026)

CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

Unbend, correction of local beam-induced sample motion in cryo-EM images using a 3D spline model.

Kong L, Zottig X, Elferich J, Grigorieff N. (2026)

Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Jones HN, Deshmukh A, Pande K. (2026)

Dual site targeting of the bacterial 70S ribosome by tetracyclines.

Devarkar SC, Lomakin IB, Wang J, Grada A, Bunick CG. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

See all citations

Protein Data Bank Japan News

[2026-07-01]

288件のPDBエントリーが新たに公開されました。（2026-07-01付）