EMPIAR-10349
Cryo Electron Tomograms of Membrane Fractions of Rabbit Skeletal Muscle for Structural Determination of RyR1 in SR Vesicles [82 tilt series in MRC format]
Publication:

Structure of RyR1 in native membranes

Chen W, Kudryashev M

EMBO reports (2020)

PMID: 32147968

Deposited:
2020-01-21
Released:
2020-03-20
Last modified:
2020-01-23
Imageset size:
254.24 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • tilt series
1. 82 tilt series (frames aligned my motioncorr2) in .st stacks (mrc format for imod) including .tlt, .xf and .defocus files
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
82
Image size:
(3710, 3838)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(2.7, 2.7)
Details:
These are tilt series which we used for generation of the 12.6 A structure or RyR1 in native membranes. the notable repeating molecules also include ATP synthase and a transporter SERCA. Details about the RYR1 structure can be found in the publication. Some tomograms have a higher exposure for the untilted micrograph which we use for "hybrid StA".

The particle folder contains 2547 unbinned CTF-corrected volumes which were used to generate the structure. The particles have not been exposure filtered. There is a Dynamo-style *.tbl which has metadata for the particles and the alignment parameters. Column 34 has class assignments for classification into 4 classes.
Files:
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Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)
Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)
Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)
Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)
Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)