EMPIAR-10349
Cryo Electron Tomograms of Membrane Fractions of Rabbit Skeletal Muscle for Structural Determination of RyR1 in SR Vesicles [82 tilt series in MRC format]
Publication:

Structure of RyR1 in native membranes

Chen W, Kudryashev M

EMBO reports (2020)

PMID: 32147968

Deposited:
2020-01-21
Released:
2020-03-20
Last modified:
2020-01-23
Imageset size:
254.24 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • tilt series
1. 82 tilt series (frames aligned my motioncorr2) in .st stacks (mrc format for imod) including .tlt, .xf and .defocus files
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
82
Image size:
(3710, 3838)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(2.7, 2.7)
Details:
These are tilt series which we used for generation of the 12.6 A structure or RyR1 in native membranes. the notable repeating molecules also include ATP synthase and a transporter SERCA. Details about the RYR1 structure can be found in the publication. Some tomograms have a higher exposure for the untilted micrograph which we use for "hybrid StA".

The particle folder contains 2547 unbinned CTF-corrected volumes which were used to generate the structure. The particles have not been exposure filtered. There is a Dynamo-style *.tbl which has metadata for the particles and the alignment parameters. Column 34 has class assignments for classification into 4 classes.
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