EMPIAR-10349 - Cryo Electron Tomograms of Membrane Fractions of Rabbit Skeletal Muscle for Structural Determination of RyR1 in SR Vesicles [82 tilt series in MRC format]

EMPIAR-10349

Cryo Electron Tomograms of Membrane Fractions of Rabbit Skeletal Muscle for Structural Determination of RyR1 in SR Vesicles [82 tilt series in MRC format]

Publication:

Structure of RyR1 in native membranes

Chen W, Kudryashev M

EMBO reports (2020)

PMID: 32147968

DOI: 10.15252/embr.201949891

Related EMDB entry:

EMD-10639 EMD-10638 EMD-10640 EMD-10637 EMD-10641 EMD-10646 EMD-10643 EMD-10642 EMD-10645 EMD-10644

Deposited:

2020-01-21

Released:

2020-03-20

Last modified:

2020-01-23

Imageset size:

254.24 GB

Imageset DOI:

10.6019/EMPIAR-10349

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. 82 tilt series (frames aligned my motioncorr2) in .st stacks (mrc format for imod) including .tlt, .xf and .defocus files

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(3710, 3838)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(2.7, 2.7)

Details:

These are tilt series which we used for generation of the 12.6 A structure or RyR1 in native membranes. the notable repeating molecules also include ATP synthase and a transporter SERCA. Details about the RYR1 structure can be found in the publication. Some tomograms have a higher exposure for the untilted micrograph which we use for "hybrid StA".

The particle folder contains 2547 unbinned CTF-corrected volumes which were used to generate the structure. The particles have not been exposure filtered. There is a Dynamo-style *.tbl which has metadata for the particles and the alignment parameters. Column 34 has class assignments for classification into 4 classes.

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Citations

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ICECREAM: high-fidelity equivariant cryo-electron tomography.

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Schäfer JH, Calza A, Hom K, Damodar P, Peng R, Bogdanović N, Lander GC, Stagg SM, Cianfrocco MA. (2025)