(1) "tv1_f01-30.mrc" contains the particles from summing frame 1 to 30. "tv1_f03-16.mrc" contains the particles from summing frame 3 to 16. All particles are boxed after motion correction. The particles are normalized (mean=0, standard deviation=1), and also contrast-inverted (white particles with black ground). "tv1_relion_data.star" is the final output of RELION auto-refine, containing all particle information.
(2) 30 particle stacks in MRC format, named as "f01.mrc", "f02.mrc", etc., store all frames of all particles. For example, "f01.mrc" contains the 1st frame of each particle, and "f02.mrc" contains the 2nd frame of each particle. These particles are boxed after motion-correction, and a direct sum should generate particles as those in "tv1_f01-30.mrc", except that the particles in "f??.mrc" are not normalized and are in original contrast (black particle with white background).
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These files are named stack_0001.mrc -> stack_0966.mrc
Files:
Available download options:
Archives and downloads the selected files into an uncompressed zip file.
Depending on the number and size of files to be downloaded, this can take an enormous amount of time.
Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.
We recommend using rsync, aspera, globus, etc.
Download a list of selected files.
It is possible to download files by specifying the file list with rsync command, etc.
These files are named stack_0001_2x_SumCorr.mrc -> stack_0966_2x_SumCorr.mrc The picked particle coordinates can be found in: micrographs/summed_frames/picked_coordinates They correspond to 3x binned images, so the values need to be multiplied by 3 to correspond to the unbinned image. The TRPV1_coords_88915 folder reflects the initial particles that were selected for further processing. The TRPV1_coords_35645 one relates to the final particles used to obtain the high-resolution map (meaning: we initially extracted 88915 particles, then post 2d and 3d classification we proceeded to minimize the stack by subselecting the final group of particles from “star” files -> the 35645 particles).
Files:
Available download options:
Archives and downloads the selected files into an uncompressed zip file.
Depending on the number and size of files to be downloaded, this can take an enormous amount of time.
Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.
We recommend using rsync, aspera, globus, etc.
Download a list of selected files.
It is possible to download files by specifying the file list with rsync command, etc.
Archives and downloads the selected files into an uncompressed zip file.
Depending on the number and size of files to be downloaded, this can take an enormous amount of time.
Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.
We recommend using rsync, aspera, globus, etc.
Download a list of selected files.
It is possible to download files by specifying the file list with rsync command, etc.