EMPIAR-10005
TRPV1 dataset taken on a K2 direct electron detector [multiple data sets in MRC format]
Publication:

Structure of the TRPV1 ion channel determined by electron cryo-microscopy

Liao M, Cao E, Julius D, Cheng Y

Nature 504 (2013) 107-112

PMID: 24305160

Related EMDB entry:
Deposited:
2013-10-25
Released:
2013-12-04
Last modified:
2016-01-19
Imageset size:
6.32 TB
Imageset DOI:
Experimental metadata:
Download xml json
Version history:
1
2015-07-13
REL
Added two datasets - raw multi-frame micrographs and summed-frame micrographs
2
2015-12-04
REL
Corrected the resolution of "TRPV1 raw multi-frame micrographs" image set to be 0.6078 ‎Å instead of 1.2156 ‎Å
3
2016-01-18
REL
Added picked_coordinates directory to micrographs
4
2016-01-19
REL
Moved picked_coordinates directory so that it is under micrographs/summed_frames and updated annotation for picked coordinates
Contains:
  • tilt series
  • micrographs - multiframe
  • micrographs - single frame
1. TRPV1 picked particles
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
35645
Image size:
(256, 256)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.2156, 1.2156)
Details:
(1)
"tv1_f01-30.mrc" contains the particles from summing frame 1 to 30.
"tv1_f03-16.mrc" contains the particles from summing frame 3 to 16.
All particles are boxed after motion correction. The particles are normalized (mean=0, standard deviation=1), and also contrast-inverted (white particles with black ground).
"tv1_relion_data.star" is the final output of RELION auto-refine, containing all particle information.

(2)
30 particle stacks in MRC format, named as "f01.mrc", "f02.mrc", etc., store all frames of all particles. For example, "f01.mrc" contains the 1st frame of each particle, and "f02.mrc" contains the 2nd frame of each particle. These particles are boxed after motion-correction, and a direct sum should generate particles as those in "tv1_f01-30.mrc", except that the particles in "f??.mrc" are not normalized and are in original contrast (black particle with white background).
Files:
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2. TRPV1 raw multi-frame micrographs
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
966
Image size:
(3710, 3710)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.6078, 0.6078)
Details:
These files are named stack_0001.mrc -> stack_0966.mrc
Files:
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3. TRPV1 summed frame micrographs
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
966
Image size:
(3710, 3710)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.2156, 1.2156)
Details:
These files are named stack_0001_2x_SumCorr.mrc -> stack_0966_2x_SumCorr.mrc
The picked particle coordinates can be found in:
micrographs/summed_frames/picked_coordinates
They correspond to 3x binned images, so the values need to be multiplied by 3 to correspond to the unbinned image.
The TRPV1_coords_88915 folder reflects the initial particles that were selected for further processing. The TRPV1_coords_35645 one relates to the final particles used to obtain the high-resolution map (meaning: we initially extracted 88915 particles, then post 2d and 3d classification we proceeded to minimize the stack by subselecting the final group of particles from “star” files -> the 35645 particles).
Files:
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Files:
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