EMPIAR-13115 - Single particle cryo-EM dataset of as-prepared Bos taurus mitochondrial complex I in MSP2N2 nanodiscs treated with NADH [13056 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-13115

Single particle cryo-EM dataset of as-prepared Bos taurus mitochondrial complex I in MSP2N2 nanodiscs treated with NADH [13056 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Post-catalysis structures of mitochondrial complex I with ubiquinol-10 bound in the active site

Chung I, Pereira CS, Wright JJ, Arantes GM, Hirst J

Nature communications (2026)

PMID: 41786699

DOI: 10.1038/s41467-026-70030-0

Related EMDB entry:

EMD-55033 EMD-55034 EMD-55031 EMD-55030 EMD-55032

Deposited:

2025-09-01

Released:

2026-03-20

Last modified:

2026-03-20

Imageset size:

5.21 TB

Imageset DOI:

10.6019/EMPIAR-13115

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of Bos taurus mitochondrial complex I from an as-prepared sample solubilised in DDM and reconstituted in to MSP2N2 nanodiscs, with 10 micromolar NADH added.

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

13056

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(None, None)

Details:

Unaligned multi-frame micrographs of Bos taurus mitochondrial complex I from an as-prepared sample solubilised in DDM and reconstituted in to MSP2N2 nanodiscs. Images (TIFF) were collected with a 300 keV FEI Titan Krios on a Falcon K3 detector, using counting mode with super-resolution mode and a Gatan energy filter (20 eV). The C2 and objective lens apertures were set to 70 and 100 µm, respectively. 2.14 s exposures were captured at a dose rate of 18.8 electrons Å−2 s−1 in 40 frames with a total dose of 40.29 electrons Å−2. A pixel size of 0.536 Å/pixel (super resolution; nominal pixel size was 1.072 Å/pixel) was used.

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Citations

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He L, Bartesaghi A. (2026)

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Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

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Schäfer JH, Calza A, Hom K, Damodar P, Peng R, Bogdanović N, Lander GC, Stagg SM, Cianfrocco MA. (2025)