EMPIAR-13115 - Single particle cryo-EM dataset of as-prepared Bos taurus mitochondrial complex I in MSP2N2 nanodiscs treated with NADH [13056 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-13115

Single particle cryo-EM dataset of as-prepared Bos taurus mitochondrial complex I in MSP2N2 nanodiscs treated with NADH [13056 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Post-catalysis structures of mitochondrial complex I with ubiquinol-10 bound in the active site

Chung I, Pereira CS, Wright JJ, Arantes GM, Hirst J

Nature communications (2026)

PMID: 41786699

DOI: 10.1038/s41467-026-70030-0

Related EMDB entry:

EMD-55033 EMD-55034 EMD-55031 EMD-55030 EMD-55032

Deposited:

2025-09-01

Released:

2026-03-20

Last modified:

2026-03-20

Imageset size:

5.21 TB

Imageset DOI:

10.6019/EMPIAR-13115

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of Bos taurus mitochondrial complex I from an as-prepared sample solubilised in DDM and reconstituted in to MSP2N2 nanodiscs, with 10 micromolar NADH added.

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

13056

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(None, None)

Details:

Unaligned multi-frame micrographs of Bos taurus mitochondrial complex I from an as-prepared sample solubilised in DDM and reconstituted in to MSP2N2 nanodiscs. Images (TIFF) were collected with a 300 keV FEI Titan Krios on a Falcon K3 detector, using counting mode with super-resolution mode and a Gatan energy filter (20 eV). The C2 and objective lens apertures were set to 70 and 100 µm, respectively. 2.14 s exposures were captured at a dose rate of 18.8 electrons Å−2 s−1 in 40 frames with a total dose of 40.29 electrons Å−2. A pixel size of 0.536 Å/pixel (super resolution; nominal pixel size was 1.072 Å/pixel) was used.

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Citations

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