EMPIAR-12615 - Cryo-EM structure of human GLUT9 apo state [multiple data sets in TIFF format]

EMPIAR-12615

Cryo-EM structure of human GLUT9 apo state [multiple data sets in TIFF format]

Publication:

Structural basis of urate transport by glucose transporter 9

Matsushita D, Toyoda Y, Lee Y, Aoi M, Matsuo H, Takada T, Nishizawa T

Cell reports 44 (2025)

PMID: 40186864

DOI: 10.1016/j.celrep.2025.115514

Related EMDB entry:

EMD-60545

Deposited:

2025-03-04

Released:

2025-06-19

Last modified:

2025-06-19

Imageset size:

4.30 TB

Imageset DOI:

10.6019/EMPIAR-12615

Experimental metadata:

Download xml json

Deposited via PDBj

Contains:

micrographs - multiframe

Image Sets

1. GLUT9-apo Imageset_1 (2022/11/03)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4967

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

- Microscope: FEI TITAN Krios
- Acceleration voltage (kV): 300
- Pixel size (A/pixel): 0.83
- Spherical aberration (Cs) : 2.7
- Defocus (nm): -800 to -1600
- Magnification:105000
- Detector: Gatan K3 BIOQUANTUM
- Average electron dose per image (e-/A2): 50
- Number of frames per image: 49
- Number of images: 4967
- Please use 'gain.mrc' as the gain reference with the flip along Y-axis for CryoSPARC (without flipping/rotation for RELION).
- Please omit the first frame, as it contains noise caused by an Digital Micrograph bug.

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2. GLUT9-apo Imageset_2 (2022/12/31)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

10255

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

- Microscope: FEI TITAN Krios
- Acceleration voltage (kV): 300
- Pixel size (A/pixel): 0.83
- Spherical aberration (Cs) : 2.7
- Defocus (nm): -800 to -1600
- Magnification:105000
- Detector: Gatan K3 BIOQUANTUM
- Average electron dose per image (e-/A2): 51
- Number of frames per image: 49
- Number of images: 10255
- Please use 'gain.tiff' as the gain reference without flipping/rotation.
- Please omit the first frame, as it contains noise caused by an Digital Micrograph bug.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. GLUT9-apo Imageset_3 (2023/01/04)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2687

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

- Microscope: FEI TITAN Krios
- Acceleration voltage (kV): 300
- Pixel size (A/pixel): 0.83
- Spherical aberration (Cs) : 2.7
- Defocus (nm): -800 to -1600
- Magnification:105000
- Detector: Gatan K3 BIOQUANTUM
- Average electron dose per image (e-/A2): 51
- Number of frames per image: 48
- Number of images: 2687
- Please use 'gain.tiff' as the gain reference without flipping/rotation.
- Please omit the first frame, as it contains noise caused by an Digital Micrograph bug.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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