- Microscope: FEI TITAN Krios - Acceleration voltage (kV): 300 - Pixel size (A/pixel): 0.83 - Spherical aberration (Cs) : 2.7 - Defocus (nm): -800 to -1600 - Magnification:105000 - Detector: Gatan K3 BIOQUANTUM - Average electron dose per image (e-/A2): 50 - Number of frames per image: 49 - Number of images: 4967 - Please use 'gain.mrc' as the gain reference with the flip along Y-axis for CryoSPARC (without flipping/rotation for RELION). - Please omit the first frame, as it contains noise caused by an Digital Micrograph bug.
- Microscope: FEI TITAN Krios - Acceleration voltage (kV): 300 - Pixel size (A/pixel): 0.83 - Spherical aberration (Cs) : 2.7 - Defocus (nm): -800 to -1600 - Magnification:105000 - Detector: Gatan K3 BIOQUANTUM - Average electron dose per image (e-/A2): 51 - Number of frames per image: 49 - Number of images: 10255 - Please use 'gain.tiff' as the gain reference without flipping/rotation. - Please omit the first frame, as it contains noise caused by an Digital Micrograph bug.
- Microscope: FEI TITAN Krios - Acceleration voltage (kV): 300 - Pixel size (A/pixel): 0.83 - Spherical aberration (Cs) : 2.7 - Defocus (nm): -800 to -1600 - Magnification:105000 - Detector: Gatan K3 BIOQUANTUM - Average electron dose per image (e-/A2): 51 - Number of frames per image: 48 - Number of images: 2687 - Please use 'gain.tiff' as the gain reference without flipping/rotation. - Please omit the first frame, as it contains noise caused by an Digital Micrograph bug.