EMPIAR-12190 - FAST-EM array tomography data of wild-type MCF-7 cells prepared with the rOTO protocol and stained with uranyl acetate [5595 micrographs in TIFF format]

EMPIAR-12190

FAST-EM array tomography data of wild-type MCF-7 cells prepared with the rOTO protocol and stained with uranyl acetate [5595 micrographs in TIFF format]

Publication:

FAST-EM array tomography: a workflow for multibeam volume electron microscopy

Kievits AJ, Duinkerken BHP, Lane R, de Heus C, van Beijeren Bergen en Henegouwen D, Höppener T, Wolters AHG, Liv N, Giepmans BNG, Hoogenboom JP

Methods in Microscopy (---)

Related EMDB entry:

Deposited:

2024-06-20

Released:

2024-09-23

Last modified:

2024-09-23

Imageset size:

561.26 GB

Imageset DOI:

10.6019/EMPIAR-12190

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Raw FAST-EM array tomography electron micrographs from wild-type MCF-7 cells stained with rOTO protocol and uranyl acetate

Category:

micrographs - single frame

Image format:

TIFF

No. of images or tilt series:

5595

Image size:

(6400, 6400)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(40.0, 40.0)

Details:

Raw serial-section (array tomography) data of MCF-7 cells acquired with FAST-EM, ordered per section. Each section directory (SXX) contains 10x10 images covering an area of approximately 240 x 240 microns, with 6.25% overlap between images. Images were acquired with 20 us dwell time.

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Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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