EMPIAR-12190
FAST-EM array tomography data of wild-type MCF-7 cells prepared with the rOTO protocol and stained with uranyl acetate [5595 micrographs in TIFF format]
Publication:

FAST-EM array tomography: a workflow for multibeam volume electron microscopy

Kievits AJ, Duinkerken BHP, Lane R, de Heus C, van Beijeren Bergen en Henegouwen D, Höppener T, Wolters AHG, Liv N, Giepmans BNG, Hoogenboom JP

Methods in Microscopy (---)

Related EMDB entry:
Deposited:
2024-06-20
Released:
2024-09-23
Last modified:
2024-09-23
Imageset size:
561.26 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - single frame
1. Raw FAST-EM array tomography electron micrographs from wild-type MCF-7 cells stained with rOTO protocol and uranyl acetate
Category:
micrographs - single frame
Image format:
TIFF
No. of images or tilt series:
5595
Image size:
(6400, 6400)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(40.0, 40.0)
Details:
Raw serial-section (array tomography) data of MCF-7 cells acquired with FAST-EM, ordered per section. Each section directory (SXX) contains 10x10 images covering an area of approximately 240 x 240 microns, with 6.25% overlap between images. Images were acquired with 20 us dwell time.
Files:
Loading...
Files:
Loading...
Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)
Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)
Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)
Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)
Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)