A dose per physical pixel/s of 5.38 was used, resulting in a total electron dose of 36.4 e-/Å2, fractionated across 172 EPU frames. These were then grouped into 26 frames, resulting in an electron dose of 0.8 e-/Å2 per frame.
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Raw movie frames are aligned with RELION's own implementation of the MotionCor2 algorithm using an EER fractionation of 45.
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