EMPIAR-11840
Structure of BARD1 ARD-BRCTs in complex with H2AKc15ub nucleosomes [multiple data sets in EER and MRC formats]
Publication:

BRCA1-BARD1 combines multiple chromatin recognition modules to bridge nascent nucleosomes

Burdett H, Foglizzo M, Musgrove LJ, Kumar D, Clifford G, Campbell LJ, Heath GR, Zeqiraj E, Wilson MD

Nucleic acids research 51 (2023) 11080-11103

PMID: 37823591

Related EMDB entry:
Deposited:
2023-08-25
Released:
2024-02-01
Last modified:
2024-02-01
Imageset size:
10.44 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
  • micrographs - single frame
1. Unaligned multiframe movies in the EER format of BARD1 ARD-BRCTs in complex with H2AKc15ub nucleosomes
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
16009
Image size:
(None, None)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(0.86, 0.86)
Details:
A dose per physical pixel/s of 5.38 was used, resulting in a total electron dose of 36.4 e-/Å2, fractionated across 172 EPU frames. These were then grouped into 26 frames, resulting in an electron dose of 0.8 e-/Å2 per frame.
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2. Aligned micrographs of BARD1 ARD-BRCTs in complex with H2AKc15ub nucleosomes
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
16009
Image size:
(None, None)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.86, 0.86)
Details:
Raw movie frames are aligned with RELION's own implementation of the MotionCor2 algorithm using an EER fractionation of 45.
Files:
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Files:
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Citations

Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)
Rigden DJ, Fernández XM. (2023)
Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)
Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)
Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)